Code No. |
MI-11-3 |
Print out |
Anti-Bromodeoxyuridine mAb
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Price |
¥48,000
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Size |
100 µL (1 mg/mL) |
Availability (in Japan) |
10 or more
(In Japan at 00:05, Mar 29, 2024 in JST)
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Clonality |
Monoclonal |
Clone |
2B1 |
Isotype
(Immunized Animal) |
Mouse IgG1 |
Applications |
IP* DNA-IP (PMID: 22850674/18708580/12944972/17671091)RNA-IP (PMID: 22369889/23064114/23383264) FCM 10 µg/mL (final concentration) IC* reported. (PMID: 19103807) IH 10 µg/mL
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Immunogen
(Antigen) |
5-Iodouridine-Ovalbumin |
Reactivity [Gene ID] |
Human |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
BrdU, 5-Bromo-2-Deoxyuridine |
Background |
BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells. |
Related products |
MI-11-5 Anti-Bromodeoxyuridine mAb-PE M075-3 Mouse IgG1 (isotype control) PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb D346-3 Anti-5-methylcytidine (m5C) mAb D347-3 Anti-Pseudouridine mAb M227-3 Anti-Digoxigenin (DIG) mAb D345-3 Anti-1-methyladenosine (m1A) mAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb RN016M Anti-7-methylguanosine (m7G)-Cap mAb RN017M Anti-7-methylguanosine (m7G) mAb PM098 Anti-Inosine pAb
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Product category |
Research area:RNA-RNP Network Epigenetics Cancer |
Data |
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Citations |
Immunoprecipitation - Katou Y et al. S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424,1078-83 (2003)(PMID:12944972)
- Bermejo R et al. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation. Genes Dev. 21, 1921-36 (2007)(PMID:17671091)
- D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
- Tani H et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012)(PMID:22369889)
- Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)
- Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID:23064114)
- Alzu A et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell 151, 835-46 (2012)(PMID:23141540)
- Tani H et al. The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013)(PMID:23383264)
- Gonzalez-Huici V et al. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. EMBO J. 33, 3667-77 (2014)(PMID:24473148)
- Toteva T et al. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. PNAS 114, 1093-1098 (2017)(PMID:28096402)
- Gay S et al. A Mad2-Mediated Translational Regulatory Mechanism Promoting S-Phase Cyclin Synthesis Controls Origin Firing and Survival to Replication Stress. Mol Cell. 70, 628-638.e5(2018)(PMID:29775579)
Flow Cytometry - Nakajima T et al. Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins. PNAS. 95,10590-5 (1998)(PMID:9724748)
- Liu Q et al. Elevated cyclin E levels, inactive retinoblastoma protein, and suppression of the p27(KIP1) inhibitor characterize early development of promyeloid cells into macrophages. Mol Cell Biol. 19, 6229-39 (1999)(PMID:10454569)
Immunocytochemistry - Ito S et al. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular.J Gen Virol. 83, 2377-83 (2002)(PMID:12237418)
- Itakura E et al. Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks. Mol Biol Cell 16, 5551-62 (2005)(PMID:16176973)
- Sugimura K et al. PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. J Cell Biol. 183, 1203-12 (2008)(PMID:19103807)
- Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)
Immunohistochemistry - Imai F et al. Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. Development. 133, 1735-44 (2006)(PMID:16571631)
- Yeom SY et al. Regulation of insulin secretion and beta-cell mass by activating signal cointegrator 2. Mol Cell Biol. 26, 4553-63 (2006)(PMID:16738321)
- Hirai S et al. The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex. J Neurosci. 26, 11992-2002 (2006)(PMID:17108173)
- Yamashita N et al. Collapsin response mediator protein 1 mediates reelin signaling in cortical neuronal migration. J Neurosci. 26, 13357-62 (2006)(PMID:17182786)
- Chen Q et al. EphA4 Regulates the Balance between Self-Renewal and Differentiation of Radial Glial Cells and Intermediate Neuronal Precursors in Cooperation with FGF Signaling. PLoS One 10, e0126942 (2015)(PMID:25978062)
- Wang D et al. spalt is functionally conserved in Locusta and Drosophila to promote wing growth. Sci Rep. 7, 44393 (2017)(PMID:28300136)
Immunofluorescence - Chen Q et al. The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo.
Front Aging Neurosci. 12, 7 (2020)(PMID:32116646)
RNP Immunoprecipitation - Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070)
Other - D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
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