Antibody detailed product information

Code No.	MD-17-3
Anti-Cyclin D1 mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:33, Apr 26, 2024 in JST）
Clonality	Monoclonal
Clone	5D4
Isotype (Immunized Animal)	Mouse IgG2a
Applications	WB　1 μg/mL IP　1-10 μg/200 μg of cell extract from 5x10⁶ cells FCM　10 μg/mL (final concentration) IH　1-10 μg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant Human PRAD1/cyclinD1
Reactivity [Gene ID]	Human, Mouse
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Product category	Research area:Cancer
Data
Citations	Western Blotting Orimoto A et al. Efficient immortalization of human dental pulp stem cells with expression of cell cycle regulators with the intact chromosomal condition. PLoS One. 15, e0229996 (2020)(PMID:32119713) Bolidong D et al. Potential therapeutic effect of targeting glycogen synthase kinase 3β in esophageal squamous cell carcinoma. Sci Rep. 10, 11807 (2020)(PMID:32678196)

The availability is based on the information in Japan at 17:33, Apr 26, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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