Antibody detailed product information

Code No. M202-3
Anti-Np95 (Uhrf1) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 16, 2024 in JST）
Clonality Monoclonal
Clone R32-44
Isotype
(Immunized Animal) 		Rat IgG2cκ
Applications WB　1 µg/mL  
IC　3 µg/mL  
IH　Can be used. (paraffin section)  
Immunogen
(Antigen)		 Recombinant protein, corresponding to amino acids 590-713 of mouse Np95 (Uhrf1)
Reactivity [Gene ID]

Human[29128], Mouse[18140]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ICBP90, RNF106, AL022808
Background DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
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Product category Research area:Epigenetics Cancer
Data



References
  1. Hashimoto H et al. The SRA domain of UHRF1 flips 5-methylcytosine out of the DNA helix. Nature 455, 826-9 (2008)
  2. Arita K et al. Recognition of hemi-methylated DNA by the SRA protein UHRF1 by a base-flipping mechanism. Nature 455, 818-21 (2008)
  3. Bostick M et al. UHRF1 plays a role in maintaining DNA methylation in mammalian cells. Science 317 1760-4 (2007)
  4. Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature 450, 908-12 (2007)
  5. Unoki M et al. ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain. Oncogene 23, 7601-10 (2007)
  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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