Antibody detailed product information

Code No. M186-7MS プリントPrint out
Anti-LC3 mAb-HRP-DirecT for My select sampler set
Price ¥9,600
Size 10 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Jan 19, 2020 in JST)
Clonality Monoclonal
Clone 8E10
Isotype
(Immunized Animal)
Mouse IgG2aκ
Applications WB 1:1,000  
Immunogen
(Antigen)
Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Reactivity [Gene ID]

Human[81631], Mouse[67443], Rat[64862], Hamster

Storage buffer PBS/Preservative/Stabilizer
Storage temp. 4°C
Conjugate HRP
Manufacturer MBL
Alternative names MAP1LC3B, MAP1ALC3
Background Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Related products PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
M186-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
Product category Research area:Autophagy
Data

Citations

Western Blotting

  1. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
  • The availability is based on the information in Japan at 00:05, Jan 19, 2020 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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