Antibody detailed product information

Code No.	M176-3
Anti-EEA1 mAb
Price	¥27,500
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Monoclonal
Clone	3C10
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB　1 µg/mL IP　2 µg/200 µL of cell extract from 3x10⁶ cells IC　1 µg/mL
Immunogen (Antigen)	Human EEA1 N-terminal synthetic peptide
Reactivity [Gene ID]	Human[8411], Mouse[216238], Rat[314764]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	MST105, ZFYVE2, MSTP105
Background	The early endosome is a cellular compartment inside eukaryotic cells. It is receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. EEA1 (Early Endosome Antigen1) is a 170 kDa coiled-coil protein, which is required for vesicular transport of proteins through early endosomes. It binds membrane lipids through its FYVE domain.
Related products	M179-3 Anti-GM130 mAb M176-A48 Anti-EEA1 mAb-Alexa Fluor™ 488 M176-A59 Anti-EEA1 mAb-Alexa Fluor™ 594 M176-A64 Anti-EEA1 mAb-Alexa Fluor™ 647 PM062 Anti-EEA1 pAb M178-3 Anti-Calnexin mAb M181-3 Anti-KDEL mAb PM059 Anti-KDEL pAb PM061 Anti-GM130 pAb
Product category	Tools:Others
Data
Citations	Immunofluorescence Itai N et al. The phosphorylation of sorting nexin 5 at serine 226 regulates retrograde transport and macropinocytosis. PLoS One. 13, e0207205 (2018)(PMID:30419003)

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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