Antibody detailed product information

Code No.	M165-3
Anti-RFP mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:00, Mar 15, 2025 in JST）
Clonality	Monoclonal
Clone	3G5
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	IP　5 µg/sample FCM　0.1-1 µg/mL IC　1 µg/mL IH*　reported. （PMID: 29575648 / 29889831）
Immunogen (Antigen)	RFP
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	Red fluorescent protein
Background	Expression vector containing a tag sequence is commonly used to introduce and express a specific gene into a target cell. Red Fluorescent Protein (RFP) fusion protein expression system is preferably used in various laboratories because of an easy monitoring of fusion proteins. This specific antibody for RFP is useful tool for monitoring of the fusion protein expression.
Related products	PM005 Anti-RFP pAb PM005-7 Anti-RFP pAb-HRP-DirecT M155-3 Anti-RFP mAb M165-8 Anti-RFP mAb-Agarose M204-3 Anti-RFP mAb M204-7 Anti-RFP mAb HRP-DirecT M208-3 Anti-RFP mAb Cocktail M165-11 Anti-RFP mAb-Magnetic Beads M165-3 Anti-RFP mAb PM073 Anti-Renilla GFP pAb
Product category	Tools:Epitope tags Fluorescent proteins
Data
Citations	Western Blotting Kobayashi T et al. Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import. Mol Biol Cell. 32, 45-56 (2020)(PMID:33175651) Immunoprecipitation Yamamoto H et al. Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4. Mol Biol Cell. 21, 2746-55 (2010)(PMID:20534812) Zhao Q et al. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Sci Rep. 7, 11250 (2017)(PMID:28900156) Immunocytochemistry Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011)(PMID:21994453) Fujimoto I et al. Necdin controls EGFR signaling linked to astrocyte differentiation in primary cortical progenitor cells. Cell Signal. 28, 94-107 (2016)(PMID:26655377) Immunohistochemistry Sumiyoshi K et al. Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109, 1513-1523 (2018)(PMID:29575648) Ojima N et al. Genetic dissection of stress-induced reproductive arrest in Drosophila melanogaster females. PLoS Genet. 14, e1007434 (2018)(PMID:29889831)

The availability is based on the information in Japan at 17:00, Mar 15, 2025 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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