Antibody detailed product information

Code No. M150-3MS プリントPrint out
Anti-Atg16L mAb for My select sampler set
Price ¥12,000
Size 20 µg/20 µL
Availability (in Japan) 1-9
（In Japan at 17:00, Jul 4, 2022 in JST）
Clonality Monoclonal
Clone 1F12
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　1 µg/mL  
IH*　reported.  （PMID: 24089213）
Immunogen
(Antigen)		 Recombinant Human ATG16L1 TV2 (85-588 a.a.)
Reactivity [Gene ID]

Human[55054], Mouse[77040], Rat[363278]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, ATG16L/WDR30, Apg16l, Atg16l, 1500009K01Rik/Wdr30, Apg16l
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Product category Research area:Autophagy
Data

Citations

Western Blotting

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
  2. Myeku N et al. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem 286, 22426-40 (2011)(PMID:24553140)
  3. Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576)
  4. Diamanti MA et al. IKKα controls ATG16L1 degradation to prevent ER stress during inflammation. J Exp Med. 214, 423-437 (2017)(PMID:28082356)

Flow Cytometry

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)

Immunocytochemistry

  1. Kucharewicz K et al. Simultaneous induction and blockade of autophagy by a single agent. Cell Death Dis. 9, 353 (2018)(PMID:29500364)
  2. Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)

Immunohistochemistry

  1. Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213)
  2. Asai E et al. Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process. Fukushima J Med Sci. 64, 15-22 (2018)(PMID:29343655)

Immunofluorescence

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)
  • The availability is based on the information in Japan at 17:00, Jul 4, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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