Antibody detailed product information

Code No. M150-3
Anti-Atg16L mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 1F12
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　1 µg/mL  
FCM*　reported.  （PMID: 25597631）
IH*　reported.  （PMID: 24089213）
Immunogen
(Antigen)		 Recombinant Human ATG16L1 TV2 (85-588 a.a.)
Reactivity [Gene ID]

Human[55054], Mouse[77040], Rat[363278]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, Apg16l
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg16L-Atg12-Atg5 forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. In recent study, nonsynonymous SNP analysis has indicated that ATG16L1 is a Crohn's disease susceptibility gene.
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Product category Research area:Autophagy
Data

Citations

Western Blotting

  1. Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213)
  2. Myeku N et al. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem 286, 22426-40 (2011)(PMID:21536669)
  3. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
  4. Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576)
  5. Diamanti MA et al. IKKα controls ATG16L1 degradation to prevent ER stress during inflammation. J Exp Med. 214, 423-437 (2017)(PMID:28082356)
  6. Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422)
  7. Shizukuishi S et al. Streptococcus pneumoniae hijacks host autophagy by deploying CbpC as a decoy for Atg14 depletion. EMBO Rep. 21, e49232 (2020)(PMID:32239622)

Immunoprecipitation

  1. Serramito-Gómez I et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun. 11, 5919 (2020)(PMID:33219218)

Flow Cytometry

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)

Immunocytochemistry

  1. Kucharewicz K et al. Simultaneous induction and blockade of autophagy by a single agent. Cell Death Dis. 9, 353 (2018)(PMID:29500364)
  2. Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)

Immunohistochemistry

  1. Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213)
  2. Asai E et al. Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process. Fukushima J Med Sci. 64, 15-22 (2018)(PMID:29343655)

Immunofluorescence

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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