Antibody detailed product information

Code No.	M150-3	Print out
Anti-Atg16L mAb
Price	¥48,000
Size	100 µg/100 µL
Availability (in Japan)	10 or more （In Japan at 00:05, Jul 8, 2020 in JST）
Clonality	Monoclonal
Clone	1F12
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	WB　1 µg/mL FCM*　reported. （PMID: 25597631） IH*　reported. （PMID: 24089213）
Immunogen (Antigen)	Recombinant Human ATG16L1 TV2 (85-588 a.a.)
Reactivity [Gene ID]	Human[55054], Mouse[77040], Rat[363278]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, Apg16l
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg16L-Atg12-Atg5 forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. In recent study, nonsynonymous SNP analysis has indicated that ATG16L1 is a Crohn's disease susceptibility gene.
Related products	8485 Autophagy Ab Sampler Set PM040 Anti-Atg16L pAb M151-3 Anti-Atg10 (Human) mAb M154-3 Anti-Atg12 (Human) mAb M153-3 Anti-Atg5 mAb PM050 Anti-Atg5 pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD017 Anti-Beclin 1 pAb PM072 Anti-VMP1 pAb PM045 Anti-p62 (SQSTM1) pAb PM040MS Anti-Atg16L pAb for My select sampler set M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb
Product category	Research area:Autophagy
Data
Citations	Western Blotting Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213) Myeku N et al. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem 286, 22426-40 (2011)(PMID:21536669) Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140) Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576) Diamanti MA et al. IKKα controls ATG16L1 degradation to prevent ER stress during inflammation. J Exp Med. 214, 423-437 (2017)(PMID:28082356) Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422) Flow Cytometry Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L⁺ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631) Immunocytochemistry Kucharewicz K et al. Simultaneous induction and blockade of autophagy by a single agent. Cell Death Dis. 9, 353 (2018)(PMID:29500364) Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087) Immunohistochemistry Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213) Asai E et al. Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process. Fukushima J Med Sci. 64, 15-22 (2018)(PMID:29343655) Immunofluorescence Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L⁺ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)

The availability is based on the information in Japan at 00:05, Jul 8, 2020 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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