Antibody detailed product information

Code No. M146-3 プリントPrint out
Anti-Nuf2 (Human) mAb
Price ¥48,000
Size 100 µg/100 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Oct 26, 2020 in JST)
Clonality Monoclonal
Clone 4H9
Isotype
(Immunized Animal)
Mouse IgG2a
Applications WB 10 µg/mL  
Immunogen
(Antigen)
Recombinant Human full-length Nuf2
Reactivity [Gene ID]

Human[83540]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names CDCA1, CT106, NUF2R
Background Nuf2, a widely conserved protein in eukaryote, compose the Ndc80 complex with NDC80/HEC1, SPC24 and SPC25. The Ndc80 complex is a constituent of the outer plate of kinetochore and acts to establish kinetochore-spindle connection at centromere for chromosome segregation in mitosis. Nuf2 localizes to kinetochores from late prophase to anaphase.
Related products D115-3 Anti-CENP-A (Human) mAb
PD030 Anti-CENP-C (Human) pAb
Product category :CancerResearch area:Cancer
Citations

Western Blotting

  1. Wu G et al. Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Mol Cell Biol. 20, 4686-95 (2009)(PMID:19776357)
  2. Ngo B et al. Complementary interhelical interactions between three buried Glu-Lys pairs within three heptad repeats are essential for Hec1-Nuf2 heterodimerization and mitotic progression. J Biol Chem. 288, 34403-13 (2013)(PMID:24129578)

Immunoprecipitation

  1. Wu G et al. Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Mol Cell Biol. 20, 4686-95 (2009)(PMID:19776357)
  • The availability is based on the information in Japan at 00:05, Oct 26, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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