Antibody detailed product information

Code No.	M137-3
Anti-HMGB1 (HMG1) mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:00, Apr 19, 2024 in JST）
Clonality	Monoclonal
Clone	4C9
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　1 µg/mL IF*　reported. （PMID: 23860260）
Immunogen (Antigen)	Human HMGB1 internal region synthetic peptide
Reactivity [Gene ID]	Human[3146], Mouse[15289], Rat[25459]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	SBP-1
Background	High mobility group box 1 (HMGB1), named for its rapid migration properties on electrophoretic gels, is a member of the nonhistone chromatin-associated proteins. HMGB1 is translated as a 214 amino acid protein, and extensively modified posttranslationally, by glycosylation, acylation, methylation, and phosphorylation. The primary structure is evolutionarily conserved, with 100% amino acid sequence homology between rat and mouse, and 99% homology between rodent and human. Intracellular HMGB1 has been studied previously for its roles in binding DNA; stabilizing nucleosome formation; as a general transcription factor for nucleolar and mitochondrial RNA polymerases; and as a gene- and tissue-specific transcriptional regulator that can enhance transcription and/or replication. Extracellular HMGB1 is recently implicated as a late mediator of delayed endotoxin lethality, because murine and human macrophages/monocytes release large amounts of a 29 kDa form of HMGB1 when stimulated by exposure to bacterial endotoxin.
Related products	D075-3 Anti-HMGB1 (HMG1) mAb PD017 Anti-Beclin 1 pAb
Product category	Research area:Epigenetics Cancer
Data
Citations	Immunofluorescence Szénási T et al. Hmgb1 can facilitate activation of the matrilin-1 gene promoter by Sox9 and L-Sox5/Sox6 in early steps of chondrogenesis. Biochim Biophs Acta 1829, 1075-91 (2013)(PMID:23860260)

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.