Antibody detailed product information

Code No.	M134-3
Anti-Atg4B mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 00:05, Jan 16, 2026 in JST）
Clonality	Monoclonal
Clone	9H5
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　1 µg/mL
Immunogen (Antigen)	Human full-length Atg4B (1-393 a.a.)
Reactivity [Gene ID]	Human[23192], Mouse[66615], Rat[316640], Hamster[100754964]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	autophagy related 4B cysteine peptidase, APG4B, AUTL1
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Atg4B is a cysteine protease required for autophagy process, which cleaves the carboxyl-terminus of LC3, to produce LC3-I. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Related products	M133-3 Anti-Atg3 mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM039 Anti-Atg7 (Human) pAb PM072 Anti-VMP1 pAb
Product category	Research area:Autophagy
Data
Citations	Western Blotting Kang YA et al. Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 32, 226-39 (2012)(PMID:22025678) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431) Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)

The availability is based on the information in Japan at 00:05, Jan 16, 2026 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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