Antibody detailed product information

Code No.	M134-3
Anti-Atg4B mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 17:00, Apr 24, 2024 in JST）
Clonality	Monoclonal
Clone	9H5
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　1 µg/mL
Immunogen (Antigen)	Human full-length Atg4B (1-393 a.a.)
Reactivity [Gene ID]	Human[23192], Mouse[66615], Rat[316640], Hamster[100754964]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	autophagy related 4B cysteine peptidase, APG4B, AUTL1
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Atg4B is a cysteine protease required for autophagy process, which cleaves the carboxyl-terminus of LC3, to produce LC3-I. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Related products	M133-3 Anti-Atg3 mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM039 Anti-Atg7 (Human) pAb PM072 Anti-VMP1 pAb
Product category	Research area:Autophagy
Data
Citations	Western Blotting Kang YA et al. Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 32, 226-39 (2012)(PMID:22025678) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431) Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)

The availability is based on the information in Japan at 17:00, Apr 24, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.