Antibody detailed product information

Code No. M084-4
Anti-Podocalyxin (PCLP1) (Human) mAb-FITC
Price ¥46,200
Size 100 µL (500 µg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 53D11
Isotype
(Immunized Animal) 		Mouse IgG2a
Applications FCM　30 μg/mL (final concentration)  
Immunogen
(Antigen)		 CHO cell expressing full-length human Podocalyxin/PCLP1
Reactivity [Gene ID]

Human[5420]
Storage buffer 500 µg/mL in PBS/1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate FITC
Manufacturer MBL
Alternative names PODXL, podocalyxin like, PC, PCLP, Gp200
Background Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin
Related products M084-3 Anti-Podocalyxin (PCLP1) (Human) mAb
M084-5 Anti-Podocalyxin (PCLP1) (Human) mAb-PE
Product category Research area:Stem cell research Cell surface antigens
Data

Citations

Flow Cytometry

  1. Kuhara M et al. Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles. Cell Struct Funct. 34, 23-30 (2009)(PMID:19293534)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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