Antibody detailed product information

Code No. K0055-3
Anti-FHL2 mAb
This clone dose not cross-react with FHL1, FHL3, FHL4 and ACT.
Price ¥33,000
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 25, 2024 in JST）
Clonality Monoclonal
Clone 11-134
Isotype
(Immunized Animal) 		Mouse IgG2a
Applications WB　1-5 µg/mL  
IP　3 µg/200-300 µL of cell extract  
IC*　reported.  （PMID: 25855776 / 26320172 / 26973248）
IH　1-5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
ChIP*　reported.  （PMID: 18378678 / 19265191 / 26025865）
Immunogen
(Antigen)		 Recombinant Human full length FHL2
Reactivity [Gene ID]

Human[2274], Mouse[14200]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names four and a half LIM domains 2, DRAL, AAG11, FHL-2, SLIM3, SLIM-3
Background Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human four-and-a-half LIM domains 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a coactivator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a transcriptional repressor in muscle cells　and is involved in modulation of β-catenin-dependent transcription of Wnt-responsive genes.
Related products 597 Anti-TRAF6 (Mouse) pAb
Product category Research area:Epigenetics Cancer
Data

Citations

Western Blotting

  1. Weinert S et al. M line-deficient titin causes cardiac lethality through impaired maturation of the sarcomere. J Cell Biol. 173, 559-70 (2006)(PMID:16702235)
  2. Kang DE et al. Presenilins mediate phosphatidylinositol 3-kinase/AKT and ERK activation via select signaling receptors. Selectivity of PS2 in platelet-derived growth factor signaling. J Biol Chem. 280, 31537-47 (2005)(PMID:16014629)
  3. Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
  4. Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
  5. Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
  6. Labalette C et al. The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways. PLoS One 3, e3761 (2008)(PMID:19018287)
  7. Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
  8. Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
  9. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  10. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)
  11. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  12. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  13. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
  14. Verset L et al. ADAM-17/FHL2 colocalisation suggests interaction and role of these proteins in colorectal cancer. Tumour Biol. 39, 1010428317695024 (2017)(PMID:28349819)
  15. Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)

ChIP

  1. Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
  2. Neuman NA et al. The four-and-a-half LIM domain protein 2 regulates vascular smooth muscle phenotype and vascular tone. J Biol Chem. 284, 13202-12 (2009)(PMID:19265191)
  3. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)

Immunoprecipitation

  1. Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
  2. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)

Immunocytochemistry

  1. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  2. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  3. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  4. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)

Immunohistochemistry

  1. Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
  2. Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
  3. Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
  4. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  5. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  6. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  7. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
  8. Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)
  • The availability is based on the information in Japan at 17:00, Apr 25, 2024 in JST.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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