Antibody detailed product information

Code No. D366-3 プリントPrint out
Anti-SaCas9 mAb
Price ¥48,000
Size 100 µg/100 µL
Availability (in Japan) 1-9
(In Japan at 17:30, Aug 10, 2022 in JST)
Clonality Monoclonal
Clone 11E7-9
Isotype
(Immunized Animal)
Mouse IgG2aκ
Applications WB 0.5 µg/mL  
IP 1 µg/sample  
IC 1 µg/mL  
Reactivity [Gene ID]

Human**, Mouse**, Rat**

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names Staphylococcus aureus Cas9, Sa Cas9
Background The CRISPR/Cas9 system is an innovative tool for genome editing. In 2015, a new Cas9 gene was cloned from Staphylococcus aureus. The SaCas9 (Staphylococcus aureus Cas9) gene is 1 kb shorter than the SpCas9 (Streptococcus pyogenes Cas9) gene, which was originally used for genome editing (Ran FA, et al., 2015, Nature). The protospacer adjacent motif (PAM) sequence of SaCas9, required for DNA cleavage, is longer than that of SpCas9. These molecular characteristics of SaCas9 are expected to enable more efficient genome editing.
Related products D366-7 Anti-SaCas9 mAb-HRP-DirecT
Notes Clone 11E7-9 does not cross-react with SpCas9 (Streptococcus pyogenes Cas9).
Product category Tools:Others
Data



References
  1. Ran FA et al. In vivo genome editing using Staphylococcus aureus Cas9. Nature 520, 186-91 (2015)(PMID: 27005865
  • The availability is based on the information in Japan at 17:30, Aug 10, 2022 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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