Antibody detailed product information

Code No. D347-3 プリントPrint out
Anti-Pseudouridine mAb
Price ¥60,000
Size 100 µg/100 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Oct 21, 2020 in JST)
Clonality Monoclonal
Clone APU-6
(Immunized Animal)
Mouse IgG1 κ
Applications IC 1 µg/mL  
IH 1 µg/mL  
ELISA* reported.  (PMID: 2758683 / 8252470)
Other(Immuno-Nothern blotting) Can be used.  (PMID: 26606401)
KLH-conjugated pseudouridine
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names 5-β-D-Ribofuranosyluracil, beta-pseudouridine, Ψ
Background Pseudouridine (ψ), the first modified RNA base to be identified, presents mostly in non-coding RNAs such as tRNA, rRNA, snRNA and snoRNA. It is also reported that pseudouridine has been found in mRNA in both yeast and human by sequencing analysis. Replacing uridine to pseudouridine enhances structural stability of RNA, and is expected to affect rRNA processing, translation and pre-mRNA splicing.
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Product category Research area:RNA-RNP Network Epigenetics


Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
  2. Mishima E and Abe T. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides. Methods Mol Biol. 1870, 179-187 (2019)(PMID:30539555)
  3. Stockert JA et al. Predictive value of pseudouridine in prostate cancer. Am J Clin Exp Urol. 7, 262-272 (2019)(PMID:31511832)
  1. Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides. Cancer 72, 3571-8 (1993)  [IHC-P, ELISA](PMID: 8252470
  2. Itoh K et al. Detection of elevated amounts of urinary pseudouridine in cancer patients by use of a monoclonal antibody. Clin Chim Acta 181, 305-15 (1989)  [ELISA](PMID: 2758683
  3. Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)(PMID: 1306318
  • The availability is based on the information in Japan at 00:05, Oct 21, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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