Antibody detailed product information

Code No. D343-3 プリントPrint out
Anti-Phospho-p62 (SQSTM1) (Ser403) mAb
Price ¥48,000
Size 100 µg/100 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Oct 24, 2019 in JST)
Clonality Monoclonal
Clone 4F6
Isotype
(Immunized Animal)
Rat IgG2aκ
Applications WB 5 µg/mL  (PMID: 22017874)
IH 10 µg/mL  (PMID: 22017874)
Immunogen
(Antigen)
Synthetic peptide CKKKESLSQMLpSMGFSDEGKKK (corresponding to amino acid residues 396-410 of human p62 (SQSTM1))
Reactivity [Gene ID]

Human[8878], Mouse[18412]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names sequestosome-1, A170, OSIL, PDB3, ZIP3
Background p62/SQSTM1 is a major adaptor protein for selective autophagy. S403 is in a highly conserved ubiquitin association (UBA) domain of p62 and is directly phosphorylated by CK2 or TBK1. The S403 phosphorylation of p62 results in the enhanced binding affinity between p62 and polyubiquitin chain and induces its autophagic degradation. S403 phosphorylated p62 is concentrated in autophagosomes that is accumulated by Bafilomycin A1 treatment and in ubiquitin positive protein aggregates in autophagy deficient cells.
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Product category Research area:Autophagy
Brands:
Data


Citations

Western Blotting

  1. Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011)(PMID:22017874)
  2. Yamada M et al. p62/SQSTM1 and Nrf2 are essential for exercise-mediated enhancement of antioxidant protein expression in oxidative muscle. FASEB J. 33, 8022-8032 (2019)(PMID:30913396)

Immunohistochemistry

  1. Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011)(PMID:22017874)
  2. Kurosawa M et al. Depletion of p62 reduces nuclear inclusions and paradoxically ameliorates disease phenotypes in Huntington's model mice. Hum Mol Genet. 24, 1092-105 (2015)(PMID:25305080)
  • The availability is based on the information in Japan at 00:05, Oct 24, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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