Antibody detailed product information

Code No. D291-3 プリントPrint out
Anti-His-tag mAb
Price ¥48,000
Size 200 µg/200 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Oct 19, 2019 in JST)
Clonality Monoclonal
Clone OGHis
Isotype
(Immunized Animal)
Mouse IgG2aκ
Applications WB 0.2 µg/mL  
IP 1 µg/sample  
FCM 0.2 µg/mL  
IC 0.5 µg/mL  
Immunogen
(Antigen)
6xHis-tagged protein
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins. This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
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Product category Tools:Epitope tags
Brands:
Data




Citations

Western Blotting

  1. Kawada J et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130, 584-92 (2012)(PMID:21413013)
  2. Suzuki T et al. Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination. Biochem Biophys Res Commun. 409, 70-4 (2011)(PMID:21549103)
  3. Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 42, 5582-93 (2014)(PMID:24711370)
  4. Ito M et al. Combinatorial contextualization of peptidic epitopes for enhanced cellular immunity. PLoS One 9, e110425 (2014)(PMID:25343355)
  5. Takeiwa T et al. Exportin-5 mediates nuclear export of SRP RNA in vertebrates. Genes Cells 20, 281-91 (2015)(PMID:25656399)
  6. Han J et al. The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. Nature 524, 243-6 (2015)(PMID:26147081)
  7. Maruyama K et al. ELISA-Based Detection System for Protein S K196E Mutation, a Genetic Risk Factor for Venous Thromboembolism. PLoS One 10, e0133196 (2015)(PMID:26186226)
  8. Li Y et al. S100A7 induction is repressed by YAP via the Hippo pathway in A431 cells. Oncotarget. 7, 38133-38142 (2016)(PMID:27203549)
  9. Ju Y et al. Arabidopsis JINGUBANG is a Negative Regulator of Pollen Germination that Prevents Pollination in Moist Environments. Plant Cell 28, 2131-2146 (2016)(PMID:27468890)
  10. Bai D et al. The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth. Nat Commun. 7, 12310 (2016)(PMID:27477389)
  11. Phadngam S et al. PTEN dephosphorylates AKT to prevent the expression of GLUT1 on plasmamembrane and to limit glucose consumption in cancer cells. Oncotarget. 7, 84999-85020 (2016)(PMID:27829222)
  12. Yan F et al. MyD88 NEDDylation negatively regulates MyD88-dependent NF-κB signaling through antagonizing its ubiquitination. Biochem Biophys Res Commun. 482, 632-637 (2017)(PMID:27864145)
  13. Endo F et al. Development of a simple and quick immunochromatography method for detection of anti-HPV-16/-18 antibodies. PLoS One. 12, e0171314 (2017)(PMID:28158224)
  14. Peng B et al. Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis. Cell Biosci. 7, 15 (2017)(PMID:28344766)
  15. Ji Y et al. Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation. Nat Commun. 8, 15308 (2017)(PMID:28516914)
  16. Lv J et al. Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation. Mol Med Rep. 16, 4475-4482 (2017)(PMID:28791409)
  17. Ahsan KB et al. SRSF1 suppresses selection of intron-distal 5′ splice site of DOK7 intron 4 to generate functional full-length Dok-7 protein. Sci Rep. 7, 10446 (2017)(PMID:28874828)
  18. Teratani T et al. Aortic carboxypeptidase-like protein, a WNT ligand, exacerbates nonalcoholic steatohepatitis. J Clin Invest. 128, 1581-1596 (2018) (PMID:29553485)
  19. Cannavo E et al. Regulatory control of DNA end resection by Sae2 phosphorylation. Nat Commun. 9, 4016 (2018)(PMID:30275497)

Co-IP

  1. Yang C et al. Ribosomal protein L6 (RPL6) is recruited to DNA damage sites in a poly(ADP-ribose) polymerase-dependent manner and regulates the DNA damage response. J Biol Chem. 294, 2827-2838 (2019)(PMID:30598506)

Immunoprecipitation

  1. Phadngam S et al. PTEN dephosphorylates AKT to prevent the expression of GLUT1 on plasmamembrane and to limit glucose consumption in cancer cells. Oncotarget. 7, 84999-85020 (2016)(PMID:27829222)

Immunocytochemistry

  1. Phadngam S et al. PTEN dephosphorylates AKT to prevent the expression of GLUT1 on plasmamembrane and to limit glucose consumption in cancer cells. Oncotarget. 7, 84999-85020 (2016)(PMID:27829222)
  • The availability is based on the information in Japan at 00:05, Oct 19, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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