Antibody detailed product information

Code No.	D278-5
Anti-GP2 (Glycoprotein 2) (Mouse) mAb-PE
Price	¥44,000
Size	1 mL
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 25, 2024 in JST）
Clonality	Monoclonal
Clone	2F11-C3
Isotype (Immunized Animal)	Rat IgG2aκ
Applications	FCM　1:10
Immunogen (Antigen)	Mouse GP2, extracellular domain (recombinant, human Fc fusion protein)
Reactivity [Gene ID]	Human(-), Mouse[67133]
Storage buffer	PBS/1% BSA/0.09% NaN₃
Storage temp.	4°C
Conjugate	PE
Manufacturer	MBL
Alternative names	glycoprotein 2 (zymogen granule membrane)
Background	M cells are located in the follicle associated epithelium (FAE) of Peyer’s patches (PPs) in the small intestine, where they mediate the uptake and transcytosis of luminal antigens to the underlying lymphoid tissue. Gycoprotein 2 (GP2) is a GPI-anchor protein that was previously thought to be exclusively expressed by pancreatic acinar cells of most mammals. Recent studies reported that GP2 is specifically expressed in M cells among intestinal epithelial cells, and serves as a transcytotic receptor for mucosal antigens. Although UEA-1 (Ulex europaeus agglutinin-1) lectin has been widely applied to detect M cells, this lectin also stains goblet cells. Furthermore, UEA-1 can only bind mouse, but not human, M cells. Thus, GP2 is the first universal M-cell marker in mouse and human. Mouse and human GP2 recombinant proteins selectively bind a subset of commensal and pathogenic enterobacteria, including Escherichia coli and Salmonella spp., by recognizing FimH-expressing type-1-pili on the bacterial outer membrane. GP2 expressed on apical surface of M cells serves as a receptor for type-1-piliated bacteria, and facilitates translocation of these bacteria from the intestinal lumen to lymphoid follicles. Consequently, antigen-specific mucosal immune response to these bacteria is induced. Thus, GP2 plays an important role in mucosal immunosurveillance. Given that M cell are considered a promising target for oral vaccination against diverse infectious diseases, the GP2-dependent transcytotic pathway could provide a new target for development of M-cell-targeted mucosal vaccines.
Related products	D277-3 Anti-GP2 (Glycoprotein 2) (Human) mAb D278-3 Anti-GP2 (Glycoprotein 2) (Mouse) mAb D278-A48 Anti-GP2 (Glycoprotein 2) (Mouse) mAb-Alexa Fluor™ 488 D278-6 Anti-GP2 (Glycoprotein 2) (Mouse) mAb-Biotin D277-A48 Anti-GP2 (Glycoprotein 2) (Human) mAb-Alexa Fluor™ 488 D277-5 Anti-GP2 (Glycoprotein 2) (Human) mAb-PE
Product category	Research area:Immunology
Data
Citations	Immunofluorescence Ramakrishnan SK et al. Intestinal non-canonical NFκB signaling shapes the local and systemic immune response. Nat Commun. 10, 660 (2019)(PMID:30737385)

The availability is based on the information in Japan at 00:05, Apr 25, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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