Antibody detailed product information

Code No. D230-3
Anti-CD274 (PD-L1) (Human) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Feb 10, 2025 in JST）
Clonality Monoclonal
Clone 27A2
Isotype
(Immunized Animal) 		Mouse IgG2b
Applications FCM　10 µg/mL (final concentration)  
IH　10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen
(Antigen)		 Recombinant Human PD-L1 extracellular domain
Reactivity [Gene ID]

Human[29126]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names B7H1
Background Programmed death ligand 1 (PD-L1, also known as CD274/B7-H1), a member of B7 family was identified by searching for molecules that share homology with the immunogloblin V and C domains of B7-1 and B7-2 among the human cDNA expressed sequence tags in the National Center for Biotechnology Information database. PD-L1 is a ligand for programmed death 1 (PD-1) which belongs to the CD28/CTLA4 subfamily. Although <em>in vitro</em> study indicated that the cross-linking of PD-1 by PD-L1 leads to down-regulation of T-cell responses, some studies have shown that T cells stimulated with low levels of anti-CD3 and immobilized PD-L1-Ig were activated, proliferation and production of IFN-&gamma; GM-CSF and IL-10 from the T cells were enhanced. The role of PD-L1 is now debatable.
Related products D230-5 Anti-CD274 (PD-L1) (Human) mAb-PE
D092-3M2 Anti-CD274 (PD-L1) (Human) mAb (Functional Grade)
D133-3M2 Anti-CD279 (PD-1) (Human) mAb (Functional Grade)
D092-3 Anti-CD274 (PD-L1) (Human) mAb
D132-3 Anti-CD279 (PD-1) (Human) mAb
D132-4 Anti-CD279 (PD-1) (Human) mAb-FITC
D132-5 Anti-CD279 (PD-1) (Human) mAb-PE
D133-5 Anti-CD279 (PD-1) (Human) mAb-PE
Product category Research area:Immunology Cancer Apoptosis Cell surface antigens
Data

Citations

Immunohistochemistry

  1. Hamanishi J et al. Programmed cell death 1 ligand 1 and tumor-infiltrating CD8+ T lymphocytes are prognostic factors of human ovarian cancer. PNAS. 104, 3360-5 (2007)(PMID:1736065)
  2. Schuchmann M et al. The programmed death (PD)-1/PD-ligand 1 pathway regulates graft-versus-host-reactive CD8 T cells after liver transplantation. Am J Transplant. 8, 2434-44 (2008)(PMID:18925909)
  3. Hino R et al. Tumor cell expression of programmed cell death-1 ligand 1 is a prognostic factor for malignant melanoma. Cancer 116, 1757-66 (2010)(PMID:20143437)
  4. Gadiot J et al. Overall survival and PD-L1 expression in metastasized malignant melanoma. Cancer 117, 2192-201 (2011)(PMID:21523733)
  5. Droeser RA et al. Clinical impact of programmed cell death ligand 1 expression in colorectal cancer. Eur J Cancer 49, 2233-42 (2013)(PMID:23478000)
  6. Hamanishi J et al. Safety and Antitumor Activity of Anti-PD-1 Antibody, Nivolumab, in Patients With Platinum-Resistant Ovarian Cancer. J Clin Oncol. 33, 4015-22 (2015)(PMID:26351349)
  7. Wu X et al. PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients. Br J Cancer. 111, 1391-9 (2014)(PMID:25093496)
  8. Goto W et al. Eribulin Promotes Antitumor Immune Responses in Patients with Locally Advanced or Metastatic Breast Cancer. Anticancer Res. 38, 2929-2938 (2018)(PMID:29715119)
  9. Ito N et al. Clinical Significance of Programmed Death Ligand-1 Expression in Esophageal Squamous Cell Carcinoma. J Surg Res. 251, 321-328 (2020)(PMID:32200323)
  • The availability is based on the information in Japan at 00:05, Feb 10, 2025 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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