Antibody detailed product information

Code No.	D204-3
Anti-CD52 (CAMPATH-1) (Mouse) mAb
Price	¥44,000
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 20, 2024 in JST）
Clonality	Monoclonal
Clone	BTG-2G
Isotype (Immunized Animal)	Rat IgG2a
Applications	IP　Can be used. FCM　10 µg/mL (final concentration) Function*　reported. （PMID: 29899181）
Immunogen (Antigen)	mouse IL-2R alpha-transgenic mouse splenocytes
Reactivity [Gene ID]	Mouse[23833]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Background	CAMPATH-1, also known as CD52, is a heavily glycosylated, GPI-anchored protein expressed at high levels on almost all thymocytes, lymphocytes, monocytes, and macrophages. The function of CD52 is unknown. However, CD52 is an exceptionally good target for complement-mediated cell lysis and antibody-mediated cellular cytotoxicity. Humanized CAMPATH-1antibodies have been used therapeutically to effectively deplete lymphocytes in allogeneic bone marrow transplants, hematologic malignancies, and autoimmune diseases. Clinical trials suggest CAMPATH-1 antibodies are especially promising in the treatment of leukemia, non-Hodgkin lymphomas, and rheumatoid arthritis. The apparent size of CAMPATH-1 by SDS-PAGE is 25-29 kDa; however, the actual molecule is much smaller, ~8-9 kDa as confirmed by total structure analysis and mass spectrometry.
Related products	D204-5 Anti-CD52 (CAMPATH-1) (Mouse) mAb-PE D204-4 Anti-CD52 (CAMPATH-1) (Mouse) mAb-FITC
Product category	Research area:Immunology Cancer Cell surface antigens
Data
Citations	Sakuragi J et al. A rapid recombination assay of HIV-1 using murine CD52 as a novel biomarker. Microbes Infect. 10, 396-404 (2008)(PMID:18407776) Function Liu J et al. Anti-mouse CD52 Treatment Ameliorates Colitis through Suppressing Th1/17 Mediated Inflammation and Promoting Tregs Differentiation in IL-10 Deficient Mice. Biol Pharm Bull. 41, 1423-1429 (2018)(PMID:29899181) Han Y et al. Genome-wide analysis highlights contribution of immune system pathways to the genetic architecture of asthma. Nat Commun. 11, 1776 (2020)(PMID:32296059)

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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