Antibody detailed product information

Code No. D199-3
Anti-MFG-E8 (Mouse) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 1-9
（In Japan at 17:33, Apr 26, 2024 in JST）
Clonality Monoclonal
Clone 18A2-G10
Isotype
(Immunized Animal) 		Hamster IgG
Applications WB　1 µg/mL  
IH　1 µg/mL (Can be used in cryosections.)  
Immunogen
(Antigen)		 Recombinant Mouse MFG-E8
Reactivity [Gene ID]

Mouse[17304]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names P47, MP47, Mfgm, SED1, lactadherin
Background Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and binds to phosphatidylserine exposed on the cell surface of apoptotic cells, then binds strongly to cells expressing avb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
Related products D161-3 Anti-MFG-E8 (Mouse) mAb
Product category Research area:Apoptosis Exosomes
Data

Citations

Western Blotting

  1. Aziz MM et al. Prolactin induces MFG-E8 production in macrophages via transcription factor C/EBPbeta-dependent pathway. Apoptosis. 13, 609-20 (2008)(PMID:18392683)
  2. Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009)(PMID:19047058)
  3. Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012)(PMID:24009879)
  4. Aziz M et al. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J immunol. 189, 393-402 (2012)(PMID:22634615)

Neutralization

  1. Fujiwara et al. The significance of tumor cells-derived MFG-E8 in tumor growth of angiosarcoma. J Dermatol Sci. 96, 18-25 (2019)(PMID:31447183)

Immunohistochemistry

  1. Jinushi M et al. MFG-E8-mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF. J Clin Invest. 117, 1902-13 (2007)(PMID:17557120)
  2. Kranich J et al. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med. 205, 1293-302 (2008)(PMID:18490487)
  3. Middendorp S et al. Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma. Blood. 114, 2280-9 (2009)(PMID:19608748)
  4. Yoshino M et al. Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. Immunol Lett. 115, 131-37 (2008)(PMID:18083239)
  5. Abe T et al. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 193, 1383-91 (2014)(PMID:24958900)
  • The availability is based on the information in Japan at 17:33, Apr 26, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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