Antibody detailed product information

Code No. D164-3
Anti-NEU3 (Human) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 1-9
（In Japan at 17:00, Apr 24, 2024 in JST）
Clonality Monoclonal
Clone 11B
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　1 μg/mL  
IP*　reported.  （PMID: 12011038 / 12730204）
IC*　reported.  （PMID: 19686243）
IH*　reported.  （PMID: 17476974）
Immunogen
(Antigen)		 Human NEU3 transfected COS cells
Reactivity [Gene ID]

Human[10825]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names SIAL3
Background Gangliosides, sialic acid-containing glycosphingolipids, are present in surface membranes of cells and play important functional roles in regulating a wide range of biological processes including cell surface interactions, cell differentiation, and transmembrane signaling. Plasma membrane-associated ganglioside sialidase (NEU3) has been suggested to play essential roles in regulation of cell surface functions because of its major localization in the plasma membrane and strict substrate preference for gangliosides, thought to play important roles in cell surface events through modulation of gangliosides.
Product category Research area:Cancer Signal transduction
Data

Citations

Western Blotting

  1. Wang Y et al. A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains. J Biol Chem. 277, 26252-9 (2002)(PMID:12011038)
  2. Wada T et al. A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells. Oncogene 26, 2483-90 (2007)(PMID:17334392)
  3. Gadhoum SZ, Sackstein R. CD15 expression in human myeloid cell differentiation is regulated by sialidase activity. Nat Chem Biol. 4, 751-7 (2008)(PMID:18953356)
  4. Wang J et al. Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a. J Neurochem. 111, 547-54 (2009)(PMID:19686243)

Immunoprecipitation

  1. Wang Y et al. A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains. J Biol Chem. 277, 26252-9 (2002)(PMID:12011038)
  2. Sasaki A et al. Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice. J Biol Chem. 278, 27896-902 (2003)(PMID:12730204)

Immunocytochemistry

  1. Wang J et al. Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a. J Neurochem. 111, 547-54 (2009)(PMID:19686243)

Immunohistochemistry

  1. Nomura H et al. Expression of NEU3 (plasma membrane-associated sialidase) in clear cell adenocarcinoma of the ovary: its relationship with T factor of pTNM classification. Oncol Res. 16, 289-97 (2006)(PMID:17476974)
  • The availability is based on the information in Japan at 17:00, Apr 24, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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