Antibody detailed product information

Code No. D162-4
Anti-Flavocytochrome b558 (Human) mAb-FITC
Price ¥33,000
Size 100 µL (500 µg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Nov 17, 2025 in JST）
Clonality Monoclonal
Clone 7D5
Isotype
(Immunized Animal) 		Mouse IgG1
Applications FCM　10 µg/mL (final concentration)  
Immunogen
(Antigen)		 Human cytochrome b rich fraction
Reactivity [Gene ID]

Human[1535/1536]
Storage buffer 500 µg/mL in PBS/1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate FITC
Manufacturer MBL
Alternative names CYBA, CYBB, p22-PHOX, cytochrome b-245 light chain, p22phox, cytochrome b light chain, p22 phagocyte B-cytochrome, cytochrome
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Related products D162-3 Anti-Flavocytochrome b558 (Human) mAb
D162-5 Anti-Flavocytochrome b558 (Human) mAb-PE
Product category Research area:Signal transduction Cellular stress
Data

Citations

Flow Cytometry

  1. Bianchi M et al. Restoration of NET formation by gene therapy in CGD controls aspergillosis. Blood 114, 2619-22 (2009)(PMID:19541821)
  2. Hayee B et al. G6PC3 mutations are associated with a major defect of glycosylation: a novel mechanism for neutrophil dysfunction. Gycobiology 21, 914-24 (2011)(PMID:21385794)
  3. Uriarte SE et al. Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst. J Immunol. 391-400 (2011)(PMID:21642540)
  4. Makni-Maalej K et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol. 189, 4657-65 (2012)(PMID:23002436)
  5. De Chiara A et al. Characterization of cytosolic proliferating cell nuclear antigen (PCNA) in neutrophils: antiapoptotic role of the monomer. J Leukoc Biol. 94, 723-31 (2013)(PMID:23825390)
  6. Sürün D et al. High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing. Mol Ther Nucleic Acids 10, 1-8 (2018)(PMID:29499925)
  7. Kiffin R et al. Anti-Leukemic Properties of Histamine in Monocytic Leukemia: The Role of NOX2. Front Oncol. 8, 218 (2018)(PMID:29967760)
  8. Kawai C et al. Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91 phox , phagocyte flavocytochrome b 558 large subunit. Microbiol Immunol. 62, 269-280 (2018) (PMID:29573449)
  • The availability is based on the information in Japan at 00:05, Nov 17, 2025 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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