Antibody detailed product information

Code No. D162-3
Anti-Flavocytochrome b558 (Human) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 17, 2024 in JST）
Clonality Monoclonal
Clone 7D5
Isotype
(Immunized Animal) 		Mouse IgG1
Applications FCM　5 µg/mL (final concentration)  
Immunogen
(Antigen)		 Human cytochrome b rich fraction
Reactivity [Gene ID]

Human[1535/1536]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names CYBA, p22-PHOX, CYBB, CGD, NOX2, IMD34, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Related products D162-4 Anti-Flavocytochrome b558 (Human) mAb-FITC
D162-5 Anti-Flavocytochrome b558 (Human) mAb-PE
Product category Research area:Signal transduction Cellular stress
Data

Citations

Flow Cytometry

  1. Li XJ et al. Leu505 of Nox2 is crucial for optimal p67phox-dependent activation of the flavocytochrome b558 during phagocytic NADPH oxidase assembly. J Leukoc Biol. 81, 238-49 (2007)(PMID:17060362)
  2.  Debeurme F et al. Regulation of NADPH oxidase activity in phagocytes: relationship between FAD/NADPH binding and oxidase complex assembly. 285, 33197-208 (2010)
     
    (PMID:25374740)
  3. Lugo Reyes SO et al. Variant of X-Linked Chronic Granulomatous Disease Revealed by a Severe Burkholderia cepacia Invasive Infection in an Infant. Case Reports Immunol. 2013, 323614 (2013)(PMID:25374740)
  4. Baba LA et al. Chronic granulomatous disease in Morocco: genetic, immunological, and clinical features of 12 patients from 10 kindreds. J Clin Immunol. 34, 452-8 (2014)(PMID:24596025)
  5. Kawai C et al. Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91 phox , phagocyte flavocytochrome b 558 large subunit. Microbiol Immunol. 62, 269-280 (2018)(PMID:29573449)
  • The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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