Antibody detailed product information

Code No. D161-3
Anti-MFG-E8 (Mouse) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 25, 2024 in JST）
Clonality Monoclonal
Clone 2422
Isotype
(Immunized Animal) 		Hamster IgG
Applications IP　5 µg/250 µL of mouse bone marrow cells culture supernatant  
FCM　5-10 µg/mL  
IH*　reported.  （PMID: 18292292）
ELISA*　reported.  （PMID: 17673941）
NT*　reported.  （PMID: 25006249）
Immunogen
(Antigen)		 thioglycollate-elicited mouse peritoneal macrophages
Reactivity [Gene ID]

Mouse[17304]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names P47, MP47, Mfgm, SED1, lactadherin
Background Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing anb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
Related products M199-3 Armenian Hamster IgG (isotype control)
D199-3 Anti-MFG-E8 (Mouse) mAb
SY-001 Anti-Fas (CD95) (Human) mAb
Product category Research area:Apoptosis
Data

Citations

Western Blotting

  1. Cui T et al. Milk fat globule epidermal growth factor 8 attenuates acute lung injury in mice after intestinal ischemia and reperfusion. Am J Respir Crit Care Med. 181, 238-46 (2010)(PMID:19892861)
  2. Zhang F et al. Milk fat globule epidermal growth factor-factor 8 mitigates inflammation and tissue injury after hemorrhagic shock in experimental animals. J Trauma Acute Care Surg. 72, 861-9 (2012)(PMID:22491597)

Neutralization

  1. Soki FN et al. Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis. J Biol Chem. 289, 24560-72 (2014)(PMID:25006249)

Immunoprecipitation

  1. Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009)(PMID:19047058)

Flow Cytometry

  1. Usui K et al. Isolation and characterization of naïve follicular dendritic cells. Mol Immunol. 50, 172-6 (2012)(PMID:22189408)

Immunohistochemistry

  1. Fens MH et al. Angiogenic endothelium shows lactadherin-dependent phagocytosis of aged erythrocytes and apoptotic cells. Blood 111, 4542-50 (2008)(PMID:18292292)

ELISA

  1. Miksa M et al. Fractalkine-induced MFG-E8 leads to enhanced apoptotic cell clearance by macrophages. Mol Med. 13, 553-60 (2007)(PMID:17673941)
  • The availability is based on the information in Japan at 17:00, Apr 25, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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