Antibody detailed product information

Code No. D153-A48 プリントPrint out
Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 488
Price ¥36,000
Size 50 µg/50 µL
Availability (in Japan) 1-9
(In Japan at 00:05, Dec 9, 2019 in JST)
Clonality Monoclonal
Clone RQ2
Isotype
(Immunized Animal)
Rat IgG2aκ
Applications IC 2-5 µg/mL  
Immunogen
(Antigen)
GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
Storage buffer 1 mg/mL in PBS/1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate Alexa Fluor® 488
Manufacturer MBL
Background Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunocytochemistry.
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Product category Tools:Epitope tags Fluorescent proteins
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Data

Citations
  1. Kitamura A et al. Different aggregation states of a nuclear localization signal-tagged 25-kDa C-terminal fragment of TAR RNA/DNA-binding protein 43 kDa. Genes Cells. 22, 521-534 (2017)(PMID:28497562)

Flow Cytometry

  1. Takashina T et al. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering. Biomaterials 173, 11-21 (2018)(PMID:29734017)

Immunocytochemistry

  1. Takashina T et al. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering. Biomaterials 173, 11-21 (2018)(PMID:29734017)
  • The availability is based on the information in Japan at 00:05, Dec 9, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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