Code No. |
D153-3 |
Anti-GFP (Green Fluorescent Protein) mAb
|
Price |
¥63,800
|
Size |
100 µL (1 mg/mL) |
Availability (in Japan) |
10 or more
(In Japan at 17:00, Apr 26, 2024 in JST)
|
Clonality |
Monoclonal |
Clone |
RQ2 |
Isotype
(Immunized Animal) |
Rat IgG2aκ |
Applications |
IP 0.5-2 µg/sample IC 2 µg/mL
|
Immunogen
(Antigen) |
GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Background |
Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Anti-GFP (Green Fluorescent Protein) mAb (clone RQ2) can detect GFP fusion protein on Immunoprecipitation and immunocytochemistry. |
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Product category |
Tools:Epitope tags Fluorescent proteins |
Data |
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Citations |
Western Blotting - Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
- Nishimura R et al. Solo and Keratin Filaments Regulate Epithelial Tubule Morphology. Cell Struct Funct. 43, 95-105 (2018)(PMID:29709890)
- Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)
Co-IP - Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012)(PMID:22801428)
Immunoprecipitation - Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004)(PMID:15502821)
- Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008)(PMID:18936252)
- Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)(PMID:23658229)
- Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
- Luteijn RD et al. Cowpox virus protein CPXV012 eludes CTLs by blocking ATP binding to TAP. J Immunol. 193, 1578-89 (2014)(PMID:25024387)
- Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019)(PMID:30482843)
Immunocytochemistry - Kunishima S et al. ACTN1 mutations cause congenital macrothrombocytopenia. Am J Hum Genet. 92, 431-8 (2013)(PMID:23434115)
- Yamanaka T et al. Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci Rep. 10, 7610 (2020)(PMID:32376919)
Immunohistochemistry - Matsuoka S et al. gone early, a novel germline factor, ensures the proper size of the stem cell precursor pool in the Drosophila ovary. PLoS One. 9, e113423 (2014)(PMID:25420147)
- Okazaki H et al. Calcineurin knockout mice show a selective loss of small spines. Neurosci Lett. 671, 99-102 (2018)(PMID:29427598)
- Urata Y et al. Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open 7, bio033142 (2018)(PMID:29903864)
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