Antibody detailed product information

Code No. D139-3 プリントPrint out
Anti-Ring1B mAb
Price ¥48,000
Size 100 µg/100 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Oct 24, 2019 in JST)
Clonality Monoclonal
Clone 3-3
Isotype
(Immunized Animal)
Mouse IgG2b
Applications WB 1 µg/mL  
IP 1-5 µg/200-300 µL of cell extract  
IH* reported.  (PMID: 20351251)
IF* reported.  (PMID: 24055057)
ChIP* reported.  (PMID: 20473294 / 21170310)
Immunogen
(Antigen)
Recombinant Mouse full length Ring1B
Reactivity [Gene ID]

Human[6045], Mouse[19821], Hamster

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names Rnf2/ring finger protein 2, BAP1, DING, BAP-1, HIPI3, RING1B/dinG
Background Polycomb-group (PcG) proteins form multimeric complexes that maintain the state of transcriptional repression of several regulatory genes during development. Ring1B/Rnf2 forms part of a protein complex containing other PcG proteins, such as Mel18, Bmi1, MBLR, MPc3, and the spliceosome protein Sap155, and these complexes associate with chromatin to regulate transcription. Ring1B may also play a role in the regulation of Hox gene expression by PcG complexes. Deletion of Ring1B activity results in gastrulation arrest and cell cycle inhibition.
Related products D138-3 Anti-Sap155 mAb
D221-3 Anti-Sap155 mAb
D210-3 Anti-Histone H2A mAb
Product category Research area:Epigenetics Cancer
Data

Citations

Western Blotting

  1. Sarcinella E et al. Monoubiquitylation of H2A.Z distinguishes its association with euchromatin or facultative heterochromatin. Mol Cell Biol. 27, 6457-68 (2007)(PMID:17636032)
  2. Wu X et al. Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acid Res. 36, 3590-9 (2008)(PMID:18460542)
  3. Wu J et al. Histone ubiquitination associates with BRCA1-dependent DNA damage response. Mol Cell Biol. 29, 849-60 (2009)(PMID:19015238)
  4. Alchanati I et al. The E3 ubiquitin-ligase Bmi1/Ring1A controls the proteasomal degradation of Top2alpha cleavage complex - a potentially new drug target. PLoS One 4, e8104 (2009)(PMID:19337376)
  5. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)
  6. Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 42, 9021-32 (2014)(PMID:25056311)
  7. Tatavosian R et al. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. J Biol Chem. 290, 28038-54 (2015)(PMID:26381410)
  8. Arora M et al. RING1A and BMI1 bookmark active genes via ubiquitination of chromatin-associated proteins. Nucleic Acids Res. 44, 2136-44 (2016)(PMID:26578590)

ChIP

  1. Landeira D et al. Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-24 (2010)(PMID:20473294)
  2. Mendenhall EM et al. GC-rich sequence elements recruit PRC2 in mammalian ES cells. PLoS Genet. 6, e1001244 (2010)(PMID:21170310)
  3. Wu X et al. Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acid Res. 36, 3590-9 (2008)(PMID:18460542)
  4. Barco R et al. The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4, e5060 (2009)(PMID:19337376)
  5. Williamson I et al. Anterior-posterior differences in HoxD chromatin topology in limb development. Development 139, 3157-67 (2012)(PMID:22872084)
  6. Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 42, 9021-32 (2014)(PMID:25056311)
  7. Vallot C et al. PRC2-independent chromatin compaction and transcriptional repression in cancer. Oncogene 34, 741-51 (2015)(PMID:24469045)
  8. Gao R et al. Bmi1 promotes erythroid development through regulating ribosome biogenesis. Stem Cells. 33, 925-38 (2015)(PMID:25385494)
  9. Tatavosian R et al. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. J Biol Chem. 290, 28038-54 (2015)(PMID:26381410)

Immunoprecipitation

  1. Barco R et al. The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4, e5060 (2009)(PMID:19337376)
  2. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)
  3. Si S et al. Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells. PLoS One. 11, e0154561 (2016)(PMID:27136092)

Immunohistochemistry

  1. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)

Immunofluorescence

  1. Casanova M et al. Heterochromatin reorganization during early mouse development requires a single-stranded noncoding transcript. Cell Rep. 4, 1156-67 (2013)(PMID:24055057)
  • The availability is based on the information in Japan at 00:05, Oct 24, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical & Biological Laboratories Co., Ltd. All Rights Reserve