Antibody detailed product information

Code No. D071-3
Anti-Multi Ubiquitin mAb
Clone FK1 has been reported to recognize K29-, K48-, K63-linked poly-ubiquitinylated and mono-ubiquitinylated proteins but not free ubiquitin (PMID:14976165, 24805825) .
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone FK1
Isotype
(Immunized Animal) 		Mouse IgM
Applications WB　1-5 µg/mL  
Immunogen
(Antigen)		 a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human, Mouse*(PMID: 24124537)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Related products D058-3 Anti-Multi Ubiquitin mAb
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CY-7053 CycLex® Poly-Ubiquitinated Protein ELISA Kit
M079-3 Mouse IgM (isotype control)
Product category Research area:Ubiquitin proteasome
Data

Citations

Western Blotting

  1. Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003)(PMID:12724408)
  2. Ledda F et al. Lrig1 is an endogenous inhibitor of Ret receptor tyrosine kinase activation, downstream signaling, and biological responses to GDNF. J Neurosci. 28, 39-49 (2008)(PMID:18171921)
  3. Umebayashi K et al. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 19, 3454-3462 (2008)(PMID:18508924)
  4. Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-196 (2009)(PMID:19216907)
  5. Zhou L, Yang H. The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR. PLoS One. 6, e23936 (2011)(PMID:21949687)
  6. Nivon M et al. NF-κB regulates protein quality control after heat stress through modulation of the BAG3-HspB8 complex. J Cell Sci. 125, 1141-51 (2012)(PMID:22302993)
  7. Pizon V et al. A MURF2B, a novel LC3-binding protein, participates with MURF2A in the switch between autophagy and ubiquitin proteasome system during differentiation of C2C12 muscle cells. PLoS One. 8, e76140 (2013)(PMID:24124537)
  8. Carrano AC et al. A Krüppel-like factor downstream of the E3 ligase WWP-1 mediates dietary-restriction-induced longevity in Caenorhabditis elegans. Nat Commun. 5, 3772 (2014)(PMID:24805825)
  9. Yahiro K et al. Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway. Cell Death Dis. 1, 15035 (2015)(PMID:27551466)
  10. Nahar S et al. A AAA ATPase Cdc48 With a Cofactor Ubx2 Facilitates Ubiquitylation of a Mitochondrial Fusion-Promoting Factor Fzo1 for Proteasomal Degradation. J Biochem. 167, 279-286(PMID:31804690)
References
  1. Morris JR, Solomon E. BRCA1 : BARD1 induces the formation of conjugated ubiquitin structures, dependent on K6 of ubiquitin, in cells during DNA replication and repair. Hum Mol Genet. 13, 807-17 (2004)（PMID: 14976165）
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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