Antibody detailed product information

Code No.	D058-8
Anti-Multi Ubiquitin mAb-Agarose
Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) .
Price	¥52,800
Size	Gel:200 µL (Slurry:400 µL)
Availability (in Japan)	10 or more （In Japan at 17:00, Apr 20, 2024 in JST）
Clonality	Monoclonal
Clone	FK2
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	IP　20 µL of gel slurry/300 µL of cell extract from 3x10⁶ cells
Immunogen (Antigen)	a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]	Human
Storage buffer	500-600 µg of anti-Multi Ubiquitin monoclonal antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry (in PBS/0.1% ProClin 150)
Storage temp.	4°C
Conjugate	Agarose
Manufacturer	MBL
Background	Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Related products	MK-11-3 Anti-Ubiquitin mAb D058-3 Anti-Multi Ubiquitin mAb M075-8 Mouse IgG1 (isotype control)-Agarose MK-12-3 Anti-Ubiquitin mAb D058-11 Anti-Multi Ubiquitin mAb-Magnetic Beads
Product category	Research area:Ubiquitin proteasome
Data
Citations	Immunoprecipitation Karunadharma PP et al. Respiratory chain protein turnover rates in mice are highly heterogeneous but strikingly conserved across tissues, ages, and treatments. FASEB J. 29, 3582-92 (2015)(PMID:25977255) Gainullin MR et al. Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells. Sci Rep. 7, 9012 (2017)(PMID:28827787) Gastou M et al. The severe phenotype of Diamond-Blackfan anemia is modulated by heat shock protein 70. Blood Adv. 1, 1959-1976 (2017)(PMID:29296843) Zachari M et al. Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform. Dev Cell. 50, 627-643 (2019)(PMID:31353311)

The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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