Antibody detailed product information

Code No. D058-3
Anti-Multi Ubiquitin mAb
Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) .
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone FK2
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　1-5 µg/mL  
IC*　reported.  （PMID: 12860994 / 15858004 / 16716190）
ELISA*　reported.  （PMID: 7588772）
IH*　reported.  （PMID: 29097807 / 29282717）
Immunogen
(Antigen)		 a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human, Mouse*(PMID: 15975924/17114435/18083104), Monkey*(PMID: 15858004), Yeast*(PMID: 12724408/15998872), Fruit fly*(PMID: 29456138)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
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Product category Research area:Autophagy Ubiquitin proteasome
Data

Citations

Western Blotting

  1. Yokosawa N et al. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. J Virol. 76, 12683-90 (2002)(PMID:12438594)
  2. Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003)(PMID:12724408)
  3. Inukai N et al. A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. J Biol Chem. 279, 8190-5 (2004)(PMID:14662762)
  4. Yamashita M et al. Ras-ERK MAPK cascade regulates GATA3 stability and Th2 differentiation through ubiquitin-proteasome pathway. J Biol Chem. 280, 29409-19 (2005)(PMID:15975924)
  5. Hwang GW et al. Overexpression of Rad23 confers resistance to methylmercury in saccharomyces cerevisiae via inhibition of the degradation of ubiquitinated proteins. Mol Pharmacol. 68, 1074-8 (2005)(PMID:15998872)
  6. Masuda Y et al. ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells. J Lipid Res. 47, 87-98 (2006)(PMID:16230742)
  7. Hosokawa H et al. Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3. J Immunol. 177, 7656-64 (2006)(PMID:17114435)
  8. Komatsu M et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131, 1149-63 (2007)(PMID:18083104)
  9. Shi W et al. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. J Biol Chem. 283, 31608-16 (2008)(PMID:18757370)
  10. Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem. 284, 12384-98 (2009)(PMID:19237541)
  11. Ebina M et al. Myeloma overexpressed 2 (Myeov2) regulates L11 subnuclear localization through Nedd8 modification. PLoS One. 8, e65285 (2013)(PMID:23776465)
  12. Furuya N et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631-41 (2014)(PMID:24451648)
  13. Ogawa M et al. Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine transferase found in Adams-Oliver syndrome. J Biol Chem. 290, 2137-49 (2015)(PMID:25488668)
  14. Sin Y et al. The C-terminal region and SUMOylation of cockayne syndrome group B protein play critical roles in transcription-coupled nucleotide excision repair. J Biol Chem. 291, 1387-97 (2016)(PMID:26620705)
  15. Takayama Y et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)
  16. Okuda K et al. CDKL5 controls postsynaptic localization of GluN2B-containing NMDA receptors in the hippocampus and regulates seizure susceptibility. Neurobiol Dis. 106, 158-170 (2017)(PMID:28688852)
  17. Joachim J et al Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy.　Curr Biol. 27, 2123-2136.e7 (2017)(PMID:28712572)
  18. Kim J et al. KLHL7 promotes TUT1 ubiquitination associated with nucleolar integrity: Implications for retinitis pigmentosa. Biochem Biophys Res Commun. 494, 220-226 (2017)(PMID:29032201)
  19. Kang D et al. The InR/Akt/TORC1 Growth-Promoting Signaling Negatively Regulates JAK/STAT Activity and Migratory Cell Fate during Morphogenesis. Dev Cell 44, 524-531.e5. (2018)(PMID:29456138)
  20. Mitsuhashi H et al. Functional domains of the FSHD-associated DUX4 protein. Biol Open 7, bio033977 (2018)(PMID:29618456)
  21. Matsumoto G et al. N-Acyldopamine induces aggresome formation without proteasome inhibition and enhances protein aggregation via p62/SQSTM1 expression. Sci Rep. 8, 9585 (2018)(PMID:29941919)
  22. Jena KK et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62‐NRF2 axis and autophagy. EMBO J. 37, e98358 (2018)(PMID:30143514)
  23. Cao Z et al. MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. Oncogene. 38, 4835-4855 (2019)(PMID:30816344)
  24. Chernyshova K et al. Glaucoma-Associated Mutations in the Optineurin Gene Have Limited Impact on Parkin-Dependent Mitophagy. Invest Ophthalmol Vis Sci. 60, 3625-3635 (2019)(PMID:31469402)
  25. Xue J et al. Acetylation of Alpha-Fetoprotein Promotes Hepatocellular Carcinoma Progression. Cancer Lett. 471, 12-26 (2020) (PMID:31811908)
  26. Asahina M et al. Ngly1-/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems. Hum Mol Genet. 29, 1635-1647 (2020)(PMID:32259258)
  27. Tsuda M et al. Tyrosyl-DNA phosphodiesterase 2 (TDP2) repairs topoisomerase 1 DNA-protein crosslinks and 3'-blocking lesions in the absence of tyrosyl-DNA phosphodiesterase 1 (TDP1). DNA Repair (Amst). 91-92, 102849 (2020)(PMID:32460231)
  28. Nakai N et al. Autophagy under glucose starvation enhances protein translation initiation in response to re-addition of glucose in C2C12 myotubes. FEBS Open Bio. 10, 2149-2156 (2020)(PMID:32882752)
  29. Sakai W et al. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair. Sci Rep. 10, 19704 (2020)(PMID:33184426)

Immunocytochemistry

  1. Yokosawa N et al. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. J Virol. 76, 12683-90 (2002)(PMID:12438594)
  2. Katoh K et al. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 278, 39104-13 (2003)(PMID:12860994)
  3. Broering TJ et al. Carboxyl-proximal regions of reovirus nonstructural protein muNS necessary and sufficient for forming factory-like inclusions. J Virol. 79, 6194-6206 (2005)(PMID:15858004)
  4. Nakamura M et al. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes. Genes Cells 11, 593-606 (2006)(PMID:16716190)
  5. Ishioka T et al. Impairment of the ubiquitin-proteasome system by cellular FLIP. Genes Cells 12, 735-44 (2007)(PMID:17573774)
  6. Komatsu M et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131, 1149-63 (2007)(PMID:18083104)
  7. Shi W et al. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. J Biol Chem. 283, 31608-16 (2008)(PMID:18757370)
  8. Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem. 284, 12384-98 (2009)(PMID:19237541)
  9. Choi UY et al. Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies. Exp Mol Med. 47, e159 (2015)(PMID:25882191)
  10. Ohka N et al. SNIPER(TACC3) induces cytoplasmic vacuolization and sensitizes cancer cells to Bortezomib. Cancer Sci. 108, 1032-1041 (2017)(PMID:28192613)
  11. Takayama Y et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)
  12. Xie X et al. Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities. J Cell Sci. 131, jcs210856 (2018)(PMID:29567855)

Immunohistochemistry

  1. Tsuji H et al. TDP-43 accelerates age-dependent degeneration of interneurons. Sci Rep. 7, 14972 (2017)(PMID:29097807)
  2. Watanabe S et al. Intracerebroventricular administration of Cystatin C ameliorates disease in SOD1-linked amyotrophic lateral sclerosis mice. J Neurochem. 145, 80-89 (2018)(PMID:29282717)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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