Antibody detailed product information

Code No. D044-3
Anti-IL-18 (Human) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 16, 2024 in JST）
Clonality Monoclonal
Clone 125-2H
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications IP　5 µg/0.5 µg recombinant Human IL-18  
IC*　（PMID: 15941925）
ELISA　Can be used.  
NT　0.1-1.0 µg/mL  
Immunogen
(Antigen)		 Recombinant Human IL-18
Reactivity [Gene ID]

Human[3606], Mouse(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
Background Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
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Product category Research area:Immunology Cytokine & Growth Factors
Data

Citations

Neutralization

  1. Sugawara S et al. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells. J Immunol. 167, 6568-75 (2001)(PMID:11714826)
  2. Tu Z et al. TLR-dependent cross talk between human Kupffer cells and NK cells. J Exp Med. 205, 233-44 (2008)(PMID:18195076)
  3. Nussbaumer O et al. DC-like cell-dependent activation of human natural killer cells by the bisphosphonate zoledronic acid is regulated by γδ T lymphocytes. Blood. 118, 2743-51 (2011)(PMID:21673342)
  4. Allam O et al. HIV induces production of IL-18 from intestinal epithelial cells that increases intestinal permeability and microbial translocation. PLoS One 13, e0194185 (2018)(PMID:29601578)
  5. Santegoets SJ et al. CD163 + cytokine-producing cDC2 stimulate intratumoral type 1 T cell responses in HPV16-induced oropharyngeal cancer. J Immunother Cancer. 8, e001053 (2020) (PMID:32771994)

Immunocytochemistry

  1. Skurk T et al. The proatherogenic cytokine interleukin-18 is secreted by human adipocytes. Eur J Endocrinol. 152, 863-8 (2005)(PMID:15941925)

ELISA

  1. Shida K et al. An alternative form of IL-18 in human blood plasma: complex formation with IgM defined by monoclonal antibodies. J Immunol. 166, 6671-9 (2001)(PMID:11359822)
  2. Okano M et al. Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis. Int Arch Allergy Immunol. 160, 275-86 (2013)(PMID:23075512)
  3. Thinwa J et al. Integrin-media,ted first signal for inflammasome activation in intestinal epithelial cells. J Immunol. 193, 1373-82 (2014)(PMID:24965773)
  4. Furman D et al. Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states. Nat Med. 23, 174-184 (2017)(PMID:28092664)
  5. Lagrange B et al. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Nat Commun. 9, 242 (2018)(PMID:29339744)
  6. Bossù P et al. Anti-inflammatory Effects of Homotaurine in Patients With Amnestic Mild Cognitive Impairment. Front Aging Neurosci. 10, 85 (2018)(PMID:30455639)
  7. Pal A et al. Inhibition of NLRP3 inflammasome activation by cell-permeable stapled peptides. Sci Rep. 9, 4913 (2019)(PMID:30894604)
  8. Kameda M et al. CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features. PLoS One. 15, e0241719 (2020)(PMID:33137121)

Function

  1. Guerville F et al. TCR-dependent sensitization of human γδ T cells to non-myeloid IL-18 in cytomegalovirus and tumor stress surveillance. Oncoimmunology 4, e1003011 (2015)(PMID:26155394)
  2. van Wilgenburg B et al. MAIT cells are activated during human viral infections. Nat Commun. 7, 11653 (2016)(PMID:27337592)
  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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