Antibody detailed product information

Code No. D032-3H
Anti-Proton Pump (H,K-ATPase β subunit) mAb
Price ¥32,000
Size 6 mL
Availability (in Japan) 1-9
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 2B6
Isotype
(Immunized Animal) 		Mouse IgG1
Applications IH　Ready for use  
Reactivity [Gene ID]

Human[496], Mouse[11945], Rat[24217], Bovine[614308]
Storage temp. 4°C
Conjugate Unlabeled
Manufacturer MBL
Background Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+,　K+-ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The　enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic a subunit and a smaller but heavily glycosylated b subunit. The a subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding　and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.
Related products D031-3 Anti-Proton Pump (H,K-ATPase α subunit) mAb
D031-3H Anti-Proton Pump (H,K-ATPase α subunit) mAb
D032-3 Anti-Proton Pump (H,K-ATPase β subunit) mAb
Notes This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c nu/+ mouse splenocyte with neonatal thymectomy-induced autoimmune gastritis.
Product category Tools:Others
Data

Citations

Western Blotting

  1. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)

Immunohistochemistry

  1. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)
  2. Fu DJ et al. Gastric Squamous-Columnar Junction Contains a Large Pool of Cancer-Prone Immature Osteopontin Responsive Lgr5- CD44+ Cells. Nat Commun. 11, 84 (2020)(PMID:31901081)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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