Antibody detailed product information

Code No. D031-3
Anti-Proton Pump (H,K-ATPase α subunit) mAb
Price ¥48,000
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 16, 2024 in JST）
Clonality Monoclonal
Clone 1H9
Isotype
(Immunized Animal) 		Mouse IgG1
Applications WB　2-10 µg/mL  
IP*　reported.  （PMID: 2472999）
IH　2-10 µg/mL  
Reactivity [Gene ID]

Human[495], Mouse[11944], Rat[24216], Bovine[521163]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ATP4A, ATP6A
Background Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+, K+-ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic a subunit and a smaller but heavily glycosylated b subunit. The a subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.
Related products D031-3H Anti-Proton Pump (H,K-ATPase α subunit) mAb
D032-3 Anti-Proton Pump (H,K-ATPase β subunit) mAb
D032-3H Anti-Proton Pump (H,K-ATPase β subunit) mAb
Product category Tools:Others
Data

Citations

Western Blotting

  1. Chandra M et al. A role for the Ca2+ channel TRPML1 in gastric acid secretion, based on analysis of knockout mice. Gasteroenterology 140, 857-67 (2011)(PMID:21111738)
  2. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)

Immunohistochemistry

  1. Kawamura YI et al. Introduction of Sd(a) carbohydrate antigen in gastrointestinal cancer cells eliminates selectin ligands and inhibits metastasis. Cancer Res. 65, 6220-7 (2005)(PMID:16024623)
  2. Mimata A et al. Loss of E-cadherin in mouse gastric epithelial cells induces signet ring-like cells, a possible precursor lesion of diffuse gastric cancer. Cancer Sci. 102, 942-50 (2011)(PMID:21276134)
  3. Becker V et al. New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal pH metry in extraesophageal gastroesophageal reflux disease. World J. Gasteroenterol. 21, 982-7 (2015)(PMID:25624734)
  4. Mitsuishi T et al. Clinicopathological characteristics of duodenal epithelial neoplasms: Focus on tumors with a gastric mucin phenotype (pyloric gland-type tumors). PLoS One. 12, e0174985 (2017)
     (PMID:28376132)
  5. Suzuki K et al. Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection. Cell Mol Gastroenterol Hepatol. 8, 119-142 (2019)(PMID:30910700)
  6. Ogawa T et al. CHAC1 overexpression in human gastric parietal cells with Helicobacter pylori infection in the secretory canaliculi. Helicobacter. 24, e12598 (2019)(PMID:31111570)
  7. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)
References
  1. Mori Y et al. Parietal cell autoantigens involved in neonatal thymectomy-induced murine autoimmune gastritis. Studies using monoclonal autoantibodies. Gasteroenterology 97, 364-75 (1989)（PMID: 2472999）
  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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