Antibody detailed product information

Code No.	563
Anti-VSV-G-tag pAb
Price	¥30,800
Size	100 µL
Availability (in Japan)	10 or more （In Japan at 00:05, Dec 14, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB　1:100 IC　1:100 IH*　reported. （PMID: 20200541 / 22298771）
Immunogen (Antigen)	YTDIEMNRLGK(VSV-G tag)-KLH
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Background	Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products	563-8 anti-VSV-G-tag pAb-Agarose PM035 Normal Rabbit IgG
Product category	Tools:Epitope tags
Data
Citations	Western Blotting Meyerhardt JA et al. Identification and characterization of neogenin, a DCC-related gene. Oncogene 14, 1129-36 (1997)(PMID:9121761) Nix SL et al. hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions. J Biol Chem. 275, 41192-200 (2000)(PMID:10993877) Immunocytochemistry Van Itallie CM, Anderson JM. Occludin confers adhesiveness when expressed in fibroblasts. J Cell Sci. 110, 1113-21 (1997)(PMID:9175707) Immunohistochemistry Page A et al. IKKbeta leads to an inflammatory skin disease resembling interface dermatitis. J Invest Dermatol. 130, 1598-610 (2010)(PMID:20200541) Nagamine S et al. Organ-specific sulfation patterns of heparan sulfate generated by extracellular sulfatases Sulf1 and Sulf2 in mice. J Biol Chem. 287, 9579-90 (2012)(PMID:22298771)

The availability is based on the information in Japan at 00:05, Dec 14, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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