Antibody detailed product information

Code No. 561
Anti-HA-tag pAb
Price ¥30,800
Size 100 µL
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit IgG
Applications WB　1:1,000  
IP　1 μL/sample  
IC　1:200  
IH*　reported.  （PMID: 28646206）
RIP*　reported.  （PMID: 27667193）
Immunogen
(Antigen)		 YPYDVPDYA (HA-tag)-KLH
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products 561-5 Anti-HA-tag pAb
561-8 Anti-HA-tag pAb-Agarose
M180-3 Anti-HA-tag mAb
M180-7 Anti-HA-tag mAb-HRP-DirecT
M180-10 Anti-HA-tag mAb-Magnetic Agarose
M180-A48 Anti-HA-tag mAb-Alexa Fluor™ 488
M180-A59 Anti-HA-tag mAb-Alexa Fluor™ 594
M180-A64 Anti-HA-tag mAb-Alexa Fluor™ 647
M132-3 Anti-HA-tag mAb
M132-10 Anti-HA-tag mAb-Magnetic Agarose
M132-11 Anti-HA-tag mAb-Magnetic Beads
M180-11 Anti-HA-tag mAb-Magnetic Beads
M180-6 Anti-HA-tag mAb-Biotin
Product category Tools:Epitope tags
Data

Citations

Western Blotting

  1. Asanuma S et al. Adult T-cell leukemia cells are characterized by abnormalities of Helios expression that promote T cell growth. Cancer Sci. 104, 1097-106 (2013)(PMID:23600753)
  2. Ochiai K et al. Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. Vet J. 197, 769-75 (2013)(PMID:23732075)
  3. Itsumura N et al. Compound Heterozygous Mutations in SLC30A2/ZnT2 Results in Low Milk Zinc Concentrations: A Novel Mechanism for Zinc Deficiency in a Breast-Fed Infant. PLoS One 8, e64045 (2013)(PMID:23741301)
  4. Nishiyama A et al. Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nature 502, 249-53 (2013)(PMID:24013172)
  5. Kato Y et al. Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines. BMC Vet Res. 13, 170 (2017)(PMID:28599655)
  6. Sato F et al. Lysyl Oxidase Enhances the Deposition of Tropoelastin through the Catalysis of Tropoelastin Molecules on the Cell Surface. Biol Pharm Bull. 40, 1646-1653 (2017)(PMID:28966236)
  7. Kawakami S et al. Functional alteration of canine isocitrate dehydrogenase 2 (IDH2) via an R174K mutation. J Vet Med Sci. 80, 85-91 (2018)(PMID:29162772)
  8. Kawakami S et al. R132 mutations in canine isocitrate dehydrogenase 1 (IDH1) lead to functional changes. Vet Res Commun. 42, 49-56 (2018)(PMID:29285579)
  9. Ohta K et al. Rab27a facilitates human parainfluenza virus type 2 growth by promoting cell surface transport of envelope proteins. Med Microbiol Immunol. 207, 141-150 (2018)(PMID:29374787)
  10. Murai N et al. Novel ubiquitin-independent nucleolar c-Myc degradation pathway mediated by antizyme 2. Sci Rep. 8, 3005 (2018)(PMID:29445227)
  11. Xu SH et al. ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell Death Dis. 9, 479 (2018)(PMID:29706618)
  12. Wang Q et al. NHERF1 inhibits beta-catenin-mediated proliferation of cervical cancer cells through suppression of alpha-actinin-4 expression. Cell Death Dis. 9, 668 (2018)(PMID:29867145)
  13. Watanabe S et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 8, 10888 (2018)(PMID:30022091)
  14. Chen M et al. TBC1D8 Amplification Drives Tumorigenesis through Metabolism Reprogramming in Ovarian Cancer. Theranostics. 9, 676-690 (2019)(PMID:30809301)
  15. Suzuki H et al. Generation of bicistronic reporter knockin mice for visualizing germ layers. Exp Anim. 68, 499-509 (2019)(PMID:31189761)
  16. Huang Y et al. Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance. Cells. 8, pii: E1264 (2019)(PMID:31623230)
  17. Miyazaki R et al. The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene. Biochem Biophys Res Commun. 525, 477-482 (2020)(PMID:32111355)
  18. Zhu S et al. An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. Nat Commun. 11, 1685 (2020)(PMID:32245947)
  19. Koshizuka T, Inoue N. Activation of c-Jun by human cytomegalovirus UL42 through JNK activation. PLoS One. 15, e0232635 (2020)(PMID:32369499)

Co-IP

  1. Xu SH et al. ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell Death Dis. 9, 479 (2018)(PMID:29706618)
  2. Watanabe S et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 8, 10888 (2018)(PMID:30022091)

Immunoprecipitation

  1. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)(PMID:23658229)
  2. Miyazaki R et al. The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene. Biochem Biophys Res Commun. 525, 477-482 (2020)(PMID:32111355)

Immunocytochemistry

  1. Ochiai K et al. Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. Vet J. 197, 769-75 (2013)(PMID:23732075)
  2. Nomura T et al. Intranuclear aggregation of mutant FUS/TLS as a molecular pathomechanism of amyotrophic lateral sclerosis. J Biol Chem. 289, 1192-202 (2014)(PMID:24280224)
  3. Kataoka H et al. Expression and subcellular localization of AT motif binding factor 1 in colon tumours. Mol Med Rep. 16, 3095-3102 (2017)(PMID:28713972)
  4. Sato F et al. Lysyl Oxidase Enhances the Deposition of Tropoelastin through the Catalysis of Tropoelastin Molecules on the Cell Surface. Biol Pharm Bull. 40, 1646-1653 (2017)(PMID:28966236)
  5. Ohta K et al. Rab27a facilitates human parainfluenza virus type 2 growth by promoting cell surface transport of envelope proteins. Med Microbiol Immunol. 207, 141-150 (2018)(PMID:29374787)
  6. Koshizuka T et al. Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases. Sci Rep. 8, 4447 (2018)(PMID:29535361)
  7. Chen IY et al. Severe Acute Respiratory Syndrome Coronavirus Viroporin 3a Activates the NLRP3 Inflammasome. Front Microbiol. 10, 50 (2019) (PMID:30761102)
  8. Kataoka H et al. Expression and subcellular localization of AT motif binding factor 1 in colon tumours. Mol Med Rep. 16, 3095-3102 (2017)(PMID:28713972)
  9. Koshizuka T, Inoue N. Activation of c-Jun by human cytomegalovirus UL42 through JNK activation. PLoS One. 15, e0232635 (2020)(PMID:32369499)

Immunohistochemistry

  1. Ohmori T et al. CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia B mice. Sci Rep. 7, 4159 (2017)(PMID:28646206)
  2. Hiramatsu N et al. ER membrane protein complex is required for the insertions of late-synthesized transmembrane helices of Rh1 in Drosophila photoreceptors. Mol Biol Cell. 30, 2890-2900 (2019)(PMID:31553680)

RNP Immunoprecipitation

  1. Kishikawa T et al. Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1. Nat Commun. 7, 13006 (2016)(PMID:27667193)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve