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HOME > Product search results > Code No. RN025P Anti-PCBP2 pAb

Code No.　RN025P

Anti-PCBP2 pAb

Price

¥66,000

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

200 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1 µg/mL  
IP
5 µg/500 µL of cell extract from 5 x 106 cells  
IC*
reported.  （PMID: 26990993）
RIP
15 µg/500 µL of cell extract from 1.5 x 107 cells  
Other(Immunodepletion)
reported.  （PMID: 22438951）
Other(eCLIP)
reported.  （PMID: 27018577）
Immunogen (Antigen) KLH conjugated synthetic peptide, corresponding to internal region of human PCBP2
Reactivity [Gene ID]

Human[5094](PMID: 22438951), Mouse[18521], Rat[363005]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names poly(rC) binding protein 2, HNRPE2, hnRNP-E2
Background Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins, characterized by their triple KH structure and by their poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also mediate protein-protein interactions within the RNP complexes. It binds specifically to polypyrimidine tracts within the 3' UTR of target mRNAs through KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are predominantly expressed in the nucleus and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent in the cytoplasm. The most characterized gene regulated by PCBP1 and PCBP2 is the a-globin gene, and the accumulated α-globin mRNA is known to account for approximately 95% of total cellular mRNA during terminal erythroid differentiation. Specific binding of PCBPs to 3′ UTR of mRNA is associated with the stabilization of a-globin mRNA. Microarray analysis of human mRNAs present in a specific subset of PCBP2-RNP complexes also showed a potential involvement of PCBP2 in transcription factor expression via association with their mRNA; further, the enrichment of PCBP2 mRNA in PCBP2-RNP complexes suggested that PCBP2 expression may be subject to posttranscriptional autoregulation.
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Citations

Western Blotting

  1. Neff AT et al. Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells. Genome Res. 1457-67 (2012)(PMID:22534399)
  2. Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951)
  2. Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070)

Other(Immunodepletion)

  1. Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Product category
Research area
RNA-RNP Network
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.