Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µg/250 µL of cell extract from 2.5 x 106 cells
 - IC*
- reported. (PMID: 24865424 / 26990993)
 - RIP
- 15 µg/500 µL of cell extract from 6 x 106 cells
 - Other(eCLIP)
- reported. (PMID: 27018577)

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide KFKGDSRSAGVPSR (46-59 a. a.)
|
Reactivity
[Gene ID] |
Human[5725], Mouse(-), Rat(-), Hamster(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
polypyrimidine tract binding protein 1, HNRNP-I, HNRNPI, HNRPI
|
Background |
The polypyrimidine tract binding protein 1 (PTBP1), a 57 kDa RNA binding protein also known as hnRNP1, has been implicated in multiple aspect of mRNA metabolism, including regulation of alternative splicing, internal ribosome entry site-driven translation, mRNA localization, and polyadenylation. Four of tandem RNA recognition motif domains give PTBP1 a strong binding activity to RNA. Posttranscriptional regulation of gene expression by PTBP1 has been reported on many genes, including CD154, insulin, VEGF and Human inducible nitric oxide synthase. |
Related products |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN052PW Anti-HNRNPC pAb RN060PW Anti-HNRNPD (AUF1) pAb RN019P Anti-HNRNPK pAb RN024P Anti-PCBP1 pAb RN025P Anti-PCBP2 pAb RN011P Anti-PTBP1 (Human) pAb RN114PW Anti-HNRNPA1 pAb
|
Citations |
Western Blotting - Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
- Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
- Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
- Nussbacher JK, Yeo GW. Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell. 69, 1005-1016.e7 (2018)(PMID:29547715)
- Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)
Immunoprecipitation - Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
Immunocytochemistry - Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
- Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
RNP Immunoprecipitation - Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
- Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
- Wang ZN et al. High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 8, 36185-36202 (2017)(PMID:28404950)
Other(eCLIP) - Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
- Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
|
Product category |
- Research area
- RNA-RNP Network
- Brands
- RiboCluster Profiler™
|