Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µg/250 µL of cell extract from 2.5 x 106 cells
 - IC*
- (PMID: 26990993 / 27068461)
 - RIP
- 15 µg/500 µL of cell extract from 1.2 x 107 cells
 - Other(eCLIP)
- reported. (PMID: 27068461 / 27108577)
 - Other(iCLIP)
- reported. (PMID: 26974154)

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide HQQQKALQSGPPQSRRK (563-579 aa)
|
Reactivity
[Gene ID] |
Human[10643], Mouse[140488], Rat(-), Hamster(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
IGF2BP3, insulin-like growth factor 2 mRNA binding protein 3, CT98, KOC1, IMP-3, VICKZ3/IMP3, Koc13, mimp3, Neilsen
|
Background |
IGF2BP3/IMP3 is identical to the KH domain containing protein overexpressed in cancer (KOC). The protein encoded by this gene was found to bind in vivo to IGF2 leader 3 and leader 4 mRNAs and H19 RNA. Binding of IMP3 to the 5' UTR of the IGF2 leader 3 mRNA represses translation of IGF2 during late development. The encoded protein contains several KH domains, which are important for RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. The growth regulating gene IMP3 has been characterized as a candidate for Silver-Russell syndrome. |
Related products |
RN1001 RIP-Assay Kit RN008P Anti-IGF2BP2 (IMP2) pAb RN007P Anti-IGF2BP1 (IMP1) pAb RN001M Anti-IGF2BP1 (IMP1) mAb RN1005 RIP-Assay Kit for microRNA
|
Citations |
Western Blotting - Ueki A et al. Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cells. PLoS One. 7, e50621 (2012)(PMID:23226335)
- Lederer M et al. The role of the oncofetal IGF2 mRNA-binding protein 3 (IGF2BP3) in cancer. Semin Cancer Biol. 29, 3-12 (2014)(PMID:25068994)
- Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA targets in human pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep 15, 1634-47 (2016)(PMID:27184842)
- JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)
Immunoprecipitation - Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA targets in human pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)
Immunocytochemistry - Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
- Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA targets in human pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831)
RNP Immunoprecipitation - Pasiliao CC et al. The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion. BMC Cancer 15, 266 (2015)(PMID:25886367)
- Ennajdaoui H et al. IGF2BP3 modulates the Interaction of invasion-associated transcripts with RISC. Cell Rep. 15, 1876-83 (2016)(PMID:27210763)
- Kim HY et al. IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor. Cancer Lett. 415, 30-39 (2018)(PMID:29217458)
Other(eCLIP) - Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA targets in human pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27108577)
Other(iCLIP) - Palanichamy JK et al. RNA-binding protein IGF2BP3 targeting of oncogenic transcripts promotes hematopoietic progenitor proliferation. J Clin Invest. 126, 1495-511 (2016)(PMID:26974154)
|
Product category |
- Research area
- RNA-RNP Network
Cancer - Brands
- RiboCluster Profiler™
|