Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µg/250 µL of cell extract from 2.5 x 106 cells
 - IC
- Can be used.
 - RIP
- 15 µg/500 µL of cell extract from 1.2 x 107 cells
 - Other(eCLIP)
- reported. (PMID: 27018577 / 27068461)
 - Other(iCLIP)
- reported. (PMID: 29245011)

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide KQQHQKGQSNQAQARRK(561-577 aa)
|
Reactivity
[Gene ID] |
Human[10642], Mouse[140486](PMID: 22215810), Rat(-), Hamster(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
insulin-like growth factor 2 mRNA binding protein 1, ZBP1, CRDBP, IMP-1, CRD-BP, VICKZ1/ Zbp1, Crdbp
|
Background |
The localization of IGF2 mRNA-binding protein 1 (IMP1) is associated with motility and the major functions of IMP1 are carried out by the phylogenetically conserved KH domains. IMP1 can affect stability, localization, and translation of its target RNAs. Binding of IMP1 to the leader 3 mRNA in the 5’-UTR of IGF2 inhibits translation from a leader 3 reporter mRNA. Aberrant IMP1 expression may interfere with c-myc regulation. Compared with copy number gains at 17q21 (IMP1 locus), gains at 8q24 (c-myc locus) were observed more frequently in tumors. Furthermore, IMP1 expression was frequently detected in tumors, implying mechanism of activation other than gene amplification. |
Related products |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN001M Anti-IGF2BP1 (IMP1) mAb RN008P Anti-IGF2BP2 (IMP2) pAb RN009P Anti-IGF2BP3 (IMP3) pAb RN048PW Anti-G3BP1 pAb
|
Citations |
Western Blotting - King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
- Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
- JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
Immunoprecipitation - Stoskus M et al. ETV6/RUNX1 transcript is a target of RNA-binding protein IGF2BP1 in t(12;21)(p13;q22)-positive acute lymphoblastic leukemia. Blood Cells Mol Dis. 57, 30-4 (2016)(PMID:26852652)
- Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
Immunocytochemistry - Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
- Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
- Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell 172, 590-604.e13 (2018)(PMID:29373831)
RNP Immunoprecipitation - Patel VL et al. Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control. Genes Dev. 26, 43-53 (2012)(PMID:22215810)
- King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
- Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
- de Vasconcellos JF et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. PNAS. 114, E5664-E5672 (2017)(PMID:28652347)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
Other(eCLIP) - Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
Other(iCLIP) - Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
|
Product category |
- Research area
- RNA-RNP Network
Neuroscience - Brands
- RiboCluster Profiler™
|