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HOME > Product search results > Code No. PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb

Code No. PM077

Anti-5-hydroxymethylcytosine (5hmC) pAb

Price

¥60,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Oct 20, 2019 in JST)

Size

100 µL

Anti-5-hydroxymethylcytosine (5hmC) pAb is useful for dot blot and hMeDIP. 5hmC is enriched in brain, liver and stem cells and may function as a regulator of gene activation.
Data
  • Western Blotting

  • Immunoprecipitation

    hMeDIP後のサンプルについて、Spike-in controlの合成DNAに特異的なプライマーを用いてreal-time qPCRを行い、合成DNAに対する%inputを算出した。

  • Immunohistochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:5,000  
IP
2 μL/sample  
IH
Can be used. (paraffin section)  
Immunogen (Antigen) BSA-conjugated 5-hydroxymethylcytidine
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names 5-hmC
Background DNA methylation is one of many critical epigenetic modifications. In most cases, cytosine (C) in DNA sequence is converted to 5-methylcytosine (5mC) with addition of a methyl group by the enzymatic activity of DNA methyltransferase. DNA methylation plays a role in the regulation of gene expression. It has been reported that abnormal DNA methylation level is involved in many diseases.

 

5-hydroxymethylcytosine (5hmC), a newly identified epigenetic modification following 5mC, is generated from oxidation of 5mC by the enzymatic activity of TET family proteins. 5hmC may function as a regulator of gene expression as well as 5mC.

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Product category
Research area
Epigenetics
Brands
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  • The availability is based on the information in Japan at 17:00, Oct 20, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.