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HOME > Product search results > Code No. PD009 Anti-rck (p54) pAb

Code No. PD009

Anti-rck (p54) pAb

Price

¥52,000

Availability (in Japan)

1-9

(In Japan at 17:30,
Aug 10, 2022 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunohistochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IC*
reported.  (PMID: 22674998 / 21109527 / 19812249
IH
1:100  
Immunogen (Antigen) synthetic peptide KLH-STARTENPVIC
Reactivity [Gene ID]

Human[1656], Mouse[13209], Rat[500988], Monkey*(PMID: 19458173)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names DDX6, HLR2
Background The lymphoma-associated rck (p54) gene product is a member of the DEAD box protein/RNA helicase family which has a variety of functions such as translation initiation, pre-mRNA splicing and ribosome assembly. Overexpression of human rck in a guinea pig cell line strongly inhibited the cell growth, and rck (p54) overexpression was found in >65% of colorectal tumours and >90% of hepatocellular carcinomas. Expression in tumors highly correlates with c-myc expression (>92%), suggesting rck (p54) may contribute to cell proliferation and carcinogenesis by increasing synthesis of c-myc protein via increased translation initiation of c-myc mRNA.
Related products RN033P Anti-TNRC6A (GW182) (Human) pAb
RN003M Anti-EIF2C2 (AGO2) (Human) mAb
Citations

Western Blotting

  1. Llorens F et al. A highly expressed miR-101 isomiR is a functional silencing small RNA. BMC Genomics. 14, 104 (2013)(PMID:23414127)
  2. Pérez-Vilaró G et al. Hepatitis C virus infection alters P-body composition but is independent of P-body granules. J Virol. 86, 8740-9 (2012)(PMID:22674998)
  3. Melemedjian OK et al. Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol. Pain 7, 70 (2011)(PMID:21936900)
  4. Yao B et al. Divergent GW182 functional domains in the regulation of translational silencing. Nucleic Acid Res. 39, 2534-47 (2011)(PMID:21131274)
  5. Broytman O et al. Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines. Neurobiol. Aging 30, 1962-74 (2009)(PMID:18378046)
  6. Pauley KM et al. Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol Cell Biol. 88, 205-12 (2010)(PMID:19918258)
  7. Lian S et al. Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies. Mol Cell Biol. 18, 3375-87 (2007)(PMID:17596515)

Immunoprecipitation

  1. Yu SF et al. The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome. PLoS Pathog. 7, e1002303 (2011)(PMID:22022269)

Immunocytochemistry

  1. Pérez-Vilaró G et al. Hepatitis C virus infection alters P-body composition but is independent of P-body granules. J Virol. 86, 8740-9 (2012)(PMID:22674998)
  2. Swetloff A et al. Dcp1-bodies in mouse oocytes. Mol Biol Cell. 20, 4951-61 (2009)(PMID:19812249)
  3. Carlos TS et al. Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells. J Gen Virol. 90, 2147-56 (2009)(PMID:19458173)
  4. Zeitelhofer M et al. Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons. J Neurosci. 28, 7555-62 (2008)(PMID:18650333)
  5. Kino Y et al. Intracellular localization and splicing regulation of FUS/TLS are variably affected by amyotrophic lateral sclerosis-linked mutations. Nucleic Acid Res. 39, 2781-98 (2011)(PMID:21109527)
  6. Moon SL, Parker R. Analysis of eIF2B bodies and their relationships with stress granules and P-bodies. Sci Rep. 8, 12264 (2018)(PMID:30115954)
Product category
Research area
RNA-RNP Network
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:30, Aug 10, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.