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HOME > Product search results > Code No. MI-11-3 Anti-Bromodeoxyuridine mAb

Code No. MI-11-3

Anti-Bromodeoxyuridine mAb



Availability (in Japan)

10 or more

(In Japan at 17:00,
Jul 3, 2022 in JST)


100 µg/100 µL

  • Immunohistochemistry

Clonality Monoclonal Clone 2B1
Isotype (Immunized Animal) Mouse IgG1
DNA-IP (PMID: 22850674/18708580/12944972/17671091)RNA-IP (PMID: 22369889/23064114/23383264)  
10 µg/mL (final concentration)  
reported.  (PMID: 19103807
10 µg/mL  
Immunogen (Antigen) 5-Iodouridine-Ovalbumin
Reactivity [Gene ID]


Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names BrdU, 5-Bromo-2-Deoxyuridine
Background BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.
Related products MI-11-5 Anti-Bromodeoxyuridine mAb-PE
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D347-3 Anti-Pseudouridine mAb
M227-3 Anti-Digoxigenin (DIG) mAb
D345-3 Anti-1-methyladenosine (m1A) mAb
M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb
M228-3 Anti-FITC mAb
RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
PM098 Anti-Inosine pAb


  1. Katou Y et al. S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424,1078-83 (2003)(PMID:12944972)
  2. Bermejo R et al. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation. Genes Dev. 21, 1921-36 (2007)(PMID:17671091)
  3. D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
  4. Tani H et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012)(PMID:22369889)
  5. Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)
  6. Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID:23064114)
  7. Alzu A et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell 151, 835-46 (2012)(PMID:23141540)
  8. Tani H et al. The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013)(PMID:23383264)
  9. Gonzalez-Huici V et al. DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. EMBO J. 33, 3667-77 (2014)(PMID:24473148)
  10. Toteva T et al. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. PNAS 114, 1093-1098 (2017)(PMID:28096402)
  11. Gay S et al. A Mad2-Mediated Translational Regulatory Mechanism Promoting S-Phase Cyclin Synthesis Controls Origin Firing and Survival to Replication Stress. Mol Cell. 70, 628-638.e5(2018)(PMID:29775579)

Flow Cytometry

  1. Nakajima T et al. Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins. PNAS. 95,10590-5 (1998)(PMID:9724748)
  2. Liu Q et al. Elevated cyclin E levels, inactive retinoblastoma protein, and suppression of the p27(KIP1) inhibitor characterize early development of promyeloid cells into macrophages. Mol Cell Biol. 19, 6229-39 (1999)(PMID:10454569)


  1. Ito S et al. Epstein-Barr virus nuclear antigen-1 is highly colocalized with interphase chromatin and its newly replicated regions in particular.J Gen Virol. 83, 2377-83 (2002)(PMID:12237418)
  2. Itakura E et al. Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks. Mol Biol Cell 16, 5551-62 (2005)(PMID:16176973)
  3. Sugimura K et al. PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA. J Cell Biol. 183, 1203-12 (2008)(PMID:19103807)
  4. Yamazaki S et al. Rif1 regulates the replication timing domains on the human genome. EMBO J. 31, 3667-77 (2012)(PMID:22850674)


  1. Imai F et al. Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. Development. 133, 1735-44 (2006)(PMID:16571631)
  2. Yeom SY et al. Regulation of insulin secretion and beta-cell mass by activating signal cointegrator 2. Mol Cell Biol. 26, 4553-63 (2006)(PMID:16738321)
  3. Hirai S et al. The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex. J Neurosci. 26, 11992-2002 (2006)(PMID:17108173)
  4. Yamashita N et al. Collapsin response mediator protein 1 mediates reelin signaling in cortical neuronal migration. J Neurosci. 26, 13357-62 (2006)(PMID:17182786)
  5. Chen Q et al. EphA4 Regulates the Balance between Self-Renewal and Differentiation of Radial Glial Cells and Intermediate Neuronal Precursors in Cooperation with FGF Signaling. PLoS One 10, e0126942 (2015)(PMID:25978062)
  6. Wang D et al. spalt is functionally conserved in Locusta and Drosophila to promote wing growth. Sci Rep. 7, 44393 (2017)(PMID:28300136)


  1. Chen Q et al. The Interaction of EphA4 With PDGFRβ Regulates Proliferation and Neuronal Differentiation of Neural Progenitor Cells in vitro and Promotes Neurogenesis in vivo. Front Aging Neurosci. 12, 7 (2020)(PMID:32116646)

RNP Immunoprecipitation

  1. Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070)


  1. D'Ambrosio C et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 22, 2215-27 (2008)(PMID:18708580)
Product category
Research area
RNA-RNP Network

  • The availability is based on the information in Japan at 17:00, Jul 3, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.