Anti-5-hydroxymethylcytosine (5hmC) mAb

Price

¥66,000

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Anti-5-hydroxymethylcytosine (5hmC) mAb is useful for dot blot and hMeDIP. 5hmC is enriched in brain, liver and stem cells and may function as a regulator of gene activation.

Data	Immunoprecipitation hMeDIP後のサンプルについて、Spike-in controlの合成DNAに特異的なプライマーを用いてreal-time qPCRを行い、合成DNAに対する%inputを算出した。 Immunohistochemistry Dot Blotting
Clonality	Monoclonal	Clone	1G10
Isotype (Immunized Animal)	Rabbit IgG
Applications	IP 1.0 μg/mL IH Can be used. (paraffin section) DB 0.2 μg/mL
Immunogen (Antigen)	BSA-conjugated 5-hydroxymethylcytidine
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	5-hmC
Background	DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
Related products	PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb MI-11-5 Anti-Bromodeoxyuridine mAb-PE D345-3 Anti-1-methyladenosine (m¹A) mAb D346-3 Anti-5-methylcytidine (m⁵C) mAb PM035 Normal Rabbit IgG PM085 Anti-ZBTB24 pAb PM086 Anti-ZBTB24 (Human) pAb M227-3 Anti-Digoxigenin (DIG) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN017M Anti-7-methylguanosine (m⁷G) mAb PM098 Anti-Inosine pAb D347-3 Anti-Pseudouridine mAb
Notes	Available in China and Japan only.
Product category	Research area Epigenetics

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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