Price
¥48,000
Availability (in Japan)
1-9
(In Japan at 17:00,
Mar 29, 2024 in JST)
Size
100 µL (1 mg/mL)
Data | |||||
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Clonality | Monoclonal | Clone | 1E6 | ||
Isotype (Immunized Animal) | Mouse IgG2b | ||||
Applications |
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Immunogen (Antigen) | mKG_N (1-168 a.a.) | ||||
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | ||||
Storage temp. | -20°C | Conjugate | Unlabeled | Manufacturer | MBL |
Alternative names | mKG_N | ||||
Background | CoralHue® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue® mKG_N fragment and CoralHue® mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don’t interact, CoralHue® mKG_N fragment and CoralHue® mKG_C fragment cannot approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue® mKG fragments spatially approach each other and the local effective concentration increases. As a result, mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions. | ||||
Related products | M149-3M Anti-monomeric Kusabira-Green C-terminal fragment mAb M168-3M Anti-monomeric Kusabira-Orange 2 mAb |
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Notes | This antibody (clone 1E6) has the epitope on CoralHue® mKG_N fragment and can detect the fusion protein with CoralHue® mKG_N specifically. | ||||
Citations |
Immunocytochemistry
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Product category |
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