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HOME > Product search results > Code No. D138-3 Anti-Sap155 mAb

Code No. D138-3

Anti-Sap155 mAb

Price

¥48,000

Availability (in Japan)

1-9

(In Japan at 17:00,
Oct 23, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 1A5
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
1 µg/mL  
Immunogen (Antigen) Recombinant Mouse Sap155
Reactivity [Gene ID]

Human[23451], Mouse[81898](20153721), Hamster

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names SF3B1, MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155
Background SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, SAP 49, 130, 145, and 155. SAP155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. SAP155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. SAP155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catalytic step II of the splicing pathway. SAP155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
Related products D221-3 Anti-Sap155 mAb
D139-3 Anti-Ring1B mAb
RN085PW Anti-U2AF1 pAb
Citations

Western Blotting

  1. Kotake et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112)
  2. Eto K et al. Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes. Biochem Biophys Res Commun. 393, 577-81 (2010)(PMID:20153721)
  3. Eto K et al. The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes. Mol Cell Biochem. 355, 217-22 (2011)(PMID:21553260)
  4. Atlasi Y et al. Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation. PLoS Genet. 9, e1003424 (2013)(PMID:23658527)
  5. Kralovicova J et al. Exon-centric regulation of ATM expression is population-dependent and amenable to antisense modification by pseudoexon targeting. Sci Rep. 6, 18741 (2016)(PMID:26732650)
  6. De Maio A et al. RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. Cell Rep. 25, 726-736.e7 (2018)(PMID:30332651)

Immunoprecipitation

  1. Eto K et al. Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes. Biochem Biophys Res Commun. 393, 577-81 (2010)(PMID:20153721)
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Oct 23, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.