Data |
Western Blotting

|
Clonality |
Monoclonal
|
Clone |
FK1 |
Isotype
(Immunized Animal)
|
Mouse IgM
|
Applications |
- WB
- 1-5 µg/mL

|
Immunogen
(Antigen) |
a crude poly-ubiquitin-lysozyme
|
Reactivity
[Gene ID] |
Human, Mouse*(PMID: 24124537) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Background |
Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules. |
Related products |
D058-3 Anti-Multi Ubiquitin mAb MK-11-3 Anti-Ubiquitin mAb MK-12-3 Anti-Ubiquitin mAb D058-8 Anti-Multi Ubiquitin mAb-Agarose CY-7053 CycLex® Poly-Ubiquitinated Protein ELISA Kit M079-3 Mouse IgM (isotype control)
|
Citations |
Western Blotting - Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003)(PMID:12724408)
- Ledda F et al. Lrig1 is an endogenous inhibitor of Ret receptor tyrosine kinase activation, downstream signaling, and biological responses to GDNF. J Neurosci. 28, 39-49 (2008)(PMID:18171921)
- Umebayashi K et al. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 19, 3454-3462 (2008)(PMID:18508924)
- Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-196 (2009)(PMID:19216907)
- Zhou L, Yang H. The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR. PLoS One. 6, e23936 (2011)(PMID:21949687)
- Nivon M et al. NF-κB regulates protein quality control after heat stress through modulation of the BAG3-HspB8 complex. J Cell Sci. 125, 1141-51 (2012)(PMID:22302993)
- Pizon V et al. A MURF2B, a novel LC3-binding protein, participates with MURF2A in the switch between autophagy and ubiquitin proteasome system during differentiation of C2C12 muscle cells. PLoS One. 8, e76140 (2013)(PMID:24124537)
- Carrano AC et al. A Krüppel-like factor downstream of the E3 ligase WWP-1 mediates dietary-restriction-induced longevity in Caenorhabditis elegans. Nat Commun. 5, 3772 (2014)(PMID:24805825)
- Yahiro K et al. Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway. Cell Death Dis. 1, 15035 (2015)(PMID:27551466)
- Nahar S et al. A AAA ATPase Cdc48 With a Cofactor Ubx2 Facilitates Ubiquitylation of a Mitochondrial Fusion-Promoting Factor Fzo1 for Proteasomal Degradation. J Biochem. 167, 279-286(PMID:31804690)
|
References |
- Morris JR, Solomon E. BRCA1 : BARD1 induces the formation of conjugated ubiquitin structures, dependent on K6 of ubiquitin, in cells during DNA replication and repair. Hum Mol Genet. 13, 807-17 (2004)(PMID: 14976165)
|
Product category |
- Research area
- Ubiquitin proteasome
|