Anti-Bcl-2 mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	83-8B
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 1 µg/mL FCM 10 µg/mL
Immunogen (Antigen)	Recombinant rat Bcl-2β
Reactivity [Gene ID]	Human[596], Mouse[12043], Rat[24224]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	B-cell CLL/lymphoma 2, BCL2, PPP1R50
Background	The Bcl-2 related genes can inhibit (Bcl-XL and Mcl-1) or induce (Bax, Bcl-Xs, Bag and Bad) apoptosis in several systems. Bad was identified as a Bcl-2 interacting protein using a yeast two-hybrid screening and l expression cloning. It has homology to Bcl-2 within the Bcl-2 homolog domains 1 and 2 (BH1 and BH2). In mammalian cells, Bad selectively heterodimerizes with Bcl-XL as well as Bcl-2, but not with other Bcl-2 family members (Bax, Bcl-Xs, Mcl-1 and A1). When Bad heterodimerized with Bcl-XL, it displaced Bax from Bcl-XL and promoted cell death.
Related products	M200-3 Anti-NRF2 mAb PD017 Anti-Beclin 1 pAb PM069 Anti-NRF2 pAb
Citations	Western Blotting Tamatani M et al. Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons. J Biol Chem. 274, 8531-8 (1999)(PMID:10085086) Tanaka T et al. Resistance to Fas-mediated apoptosis in human lung fibroblast. Eur Respir J. 20, 359-68 (2002)(PMID:12212968) Rincheval V et al. Bcl-2 can promote p53-dependent senescence versus apoptosis without affecting the G1/S transition. Biochem Biophys Res Commun. 298, 282-8 (2002)(PMID:12387829) Wang Y et al. Deletion of INMAP postpones mitotic exit and induces apoptosis by disabling the formation of mitotic spindle. Biochem Biophys Res Commun. 518, 19-25 (2019)(PMID:31405563) Liu TH et al. Verification of Resveratrol Inhibits Intestinal Aging by Downregulating ATF4/Chop/Bcl-2/Bax Signaling Pathway: Based on Network Pharmacology and Animal Experiment. Front Pharmacol. 11, 1064 (2020)(PMID:32754039)
Product category	Research area Apoptosis

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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