Background |
Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively. |
Citations |
Western Blotting - Matsushita T et al. Functional SNP of ARHGEF10 confers risk of atherothrombotic stroke. Hum Mol Genet. 19, 1137-46 (2010)(PMID:20042462)
- Xue JF et al. DEDD negatively regulates transforming growth factor-beta1 signaling by interacting with Smad3. FEBS Lett. 584, 3028-34 (2010)(PMID:20553715)
- Bai N et al. Dock3 interaction with a glutamate-receptor NR2D subunit protects neurons from excitotoxicity. Mol Brain 6, 22 (2013)(PMID:23641686)
- Sato M et al. Development and validation of scFv-conjugated affinity silk protein for specific detection of carcinoembryonic antigen. Sci Rep. 7, 16077 (2017)(PMID:29167497)
- Komiya M et al. A genetic screen to discover SUMOylated proteins in living mammalian cells. Sci Rep. 7, 17443 (2017)(PMID:29234079)
- Baba K et al. Gradient-reading and mechano-effector machinery for netrin-1-induced axon guidance. Elife 7, e34593 (2018)(PMID:30082022)
- Wang H et al. A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. J Exp Bot. 69, 5573-5586 (2018)(PMID:30137408)
- Rasila T et al. Astroprincin (FAM171A1, C10orf38): A Regulator of Human Cell Shape and Invasive Growth. Am J Pathol. 189, 177-189 (2019)(PMID:30312582)
- Sun P et al. A Mosquito Salivary Protein Promotes Flavivirus Transmission by Activation of Autophagy. Nat Commun. 11, 260 (2020)(PMID:31937766)
- Nishida T, Yamada Y. RNF4-mediated SUMO-targeted ubiquitination relieves PARIS/ZNF746-mediated transcriptional repression. Biochem Biophys Res Commun. 526, 110-116 (2020)(PMID:32197837)
- Hitomi S et al. Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells. In Vivo. 34, 2437-2441 (2020)
(PMID: 32871770)
ChIP - Isogai E et al. Oncogenic LMO3 collaborates with HEN2 to enhance neuroblastoma cell growth through transactivation of Mash1. PLoS One 6, e19297 (2011)(PMID:21573214)
Immunoprecipitation - Xue JF et al. DEDD negatively regulates transforming growth factor-beta1 signaling by interacting with Smad3. FEBS Lett. 584, 3028-34 (2010)(PMID:20553715)
Flow Cytometry - Gomi F et al. Up-regulation of NSP3 by Oligomeric Aβ Accelerates Neuronal Death Through Cas-independent Rap1A Activation. Neuroscience 386, 182-193 (2018)(PMID:29966723)
Immunocytochemistry - Fukunaka A et al. Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway. J Biol Chem. 286, 16363-73 (2011)(PMID:21402707)
- Nakamura S et al. Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome. Mol Genet Metab Rep. 10, 67-74 (2017)(PMID:28119822)
Immunohistochemistry - Nakaya Y et al. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 10, 765-75 (2008)(PMID:18552836)
- Shin M et al. Notch mediates Wnt and BMP signals in the early separation of smooth muscle progenitors and blood/endothelial common progenitors. Development 136, 595-603 (2009)(PMID:19168675)
- Hiramatsu N et al. ER membrane protein complex is required for the insertions of late-synthesized transmembrane helices of Rh1 in Drosophila photoreceptors. Mol Biol Cell. 30, 2890-2900 (2019)(PMID:31553680)
- Watanabe K et al. The fornix acts as a permissive corridor for septal neuron migration beyond the diencephalic-telencephalic boundary. Sci Rep. 10, 8315 (2020)(PMID:32433594)
ELISA - Sangawa T et al. A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein. Protein Sci. 22, 840-50 (2013)(PMID:23526492)
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