Anti-Glypican 1 (Human) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Jan 24, 2025 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	12C1E2F
Isotype (Immunized Animal)	Mouse IgG1κ
Applications	FCM 1-10 µg/mL
Immunogen (Antigen)	Human Glypican 1 expressed Ba/F3transfectants generated from SST-REX (signal sequence trap by retrovirus-mediated expression screening).
Reactivity [Gene ID]	Human[2817]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	GPC1
Background	Glypican 1 (GPC1) is a member of cell surface heparan sulfate proteoglycan family. GPC1 is a GPI-anchored type membrane protein and probably involves in the control of cell division and growth regulation. GPC1 also associates with fibrillar APP A-beta peptides in lipid rafts in Alzheimer disease brains. In pancreatic ductal adenocarcinoma, enhanced GPC1 expression is suggested to correlate with BMP and activin receptors. Down-regulation of GPC1 results in suppression of pancreatic cancer cell growth.
Related products	W164-3 Anti-RHBDD3 (Human) mAb M075-3 Mouse IgG1 (isotype control) MTG-001 Clear Back (Human Fc receptor blocking reagent)
References	Kojima T, Kitamura T. A signal sequence trap based on a constitutively active cytokine receptor. Nat Biotechnol. 17, 487-90 (1999)（PMID: 10331810） Cheng F et al. Suppression of amyloid beta A11 antibody immunoreactivity by vitamin C: possible role of heparan sulfate oligosaccharides derived from glypican-1 by ascorbate-induced, nitric oxide (NO)-catalyzed degradation. J Biol Chem. 286, 27559-72 (2011)（PMID: 21642435） Svensson G, Mani K. S-Nitrosylation of secreted recombinant human glypican-1. Glycoconj J. 26, 1247-57 (2009)（PMID: 19479373） Mani K et al. Constitutive and vitamin C-induced, NO-catalyzed release of heparan sulfate from recycling glypican-1 in late endosomes. Glycobiology 16, 1251-61 (2006)（PMID: 16971378） Watanabe N et al. Glypican-1 as an Abeta binding HSPG in the human brain: its localization in DIG domains and possible roles in the pathogenesis of Alzheimer's disease. FASEB J. 18, 1013-5 (2004)（PMID: 15084524） Vermeesch SA et al. Assignment of the human glypican gene (GPC1) to 2q35-q37 by fluorescence in situ hybridization. Genomics 25, 327-9 (1995)（PMID: 7774946） David G et al. Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts. J Cell Biol. 111, 3165-76 (1990)（PMID: 2148568）
Product category	Research area Cancer Tools Flow cytometry reagents

The availability is based on the information in Japan at 00:05, Jan 24, 2025 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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